PMID- 19617256
OWN - NLM
STAT- MEDLINE
DCOM- 20091214
LR  - 20221207
IS  - 1460-2423 (Electronic)
IS  - 0959-6658 (Linking)
VI  - 19
IP  - 10
DP  - 2009 Oct
TI  - The second bovine beta-galactoside-alpha2,6-sialyltransferase (ST6Gal II): 
      genomic organization and stimulation of its in vitro expression by IL-6 in bovine 
      mammary epithelial cells.
PG  - 1082-93
LID - 10.1093/glycob/cwp094 [doi]
AB  - We have cloned a cDNA sequence encoding the second bovine 
      beta-galactoside-alpha2,6-sialyltransferase whose sequence shares more than 75% 
      of identity with hST6Gal II cDNA coding sequence. The bovine gene, located on BTA 
      11, spans over 50 kbp with five exons (E1-E5) containing the 1488 bp open reading 
      frame and a 5'-untranslated exon (E0). The gene expression pattern reveals a 
      specific tissue distribution (brain, lungs, spleen, salivary, and mammary glands) 
      compared to ST6Gal I which is ubiquitously expressed. We identified for bovine 
      ST6Gal II three kinds of transcripts which differ by their 5'-untranslated 
      regions. Among them, two transcripts are brain specific whereas the third one is 
      found in all of the tissues expressing the gene. Two pFlag-bST6Gal II vector 
      constructions were separately transfected in COS-1 cells in order to express 
      either membrane-bound or soluble active forms of ST6Gal II. Enzymatic assays with 
      these two forms indicated that the enzyme used the LacdiNAc structure 
      (GalNAcbeta1,4GlcNAc) as a better acceptor substrate than the Type II 
      (Galbeta1-4GlcNAc) disaccharide. Moreover, the enzyme's efficiency is improved 
      when the acceptor substrate is provided as a free oligosaccharide rather than as 
      a protein-bound oligosaccharide. In order to investigate the potential role of 
      ST6Gal II during the acute phase of inflammation, we used primary cultures of 
      bovine mammary epithelial cells which were stimulated with pro-inflammatory 
      cytokines. It appears that the ST6Gal II gene was upregulated in cells stimulated 
      by IL-6. This result suggested that alpha2,6-sialylation mediated by this gene 
      could contribute to organism's response to infections.
FAU - Laporte, Benoit
AU  - Laporte B
AD  - UMR1061, Unite de Genetique Moleculaire Animale, Universite de Limoges, INRA, IFR 
      N degrees 145 GEIST, France.
FAU - Gonzalez-Hilarion, Sara
AU  - Gonzalez-Hilarion S
FAU - Maftah, Abderrahman
AU  - Maftah A
FAU - Petit, Jean-Michel
AU  - Petit JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090717
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-6)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.1 (beta-D-Galactoside alpha 2-6-Sialyltransferase)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Epithelial Cells/*metabolism
MH  - Female
MH  - Gene Expression/*drug effects
MH  - *Genome
MH  - Humans
MH  - Interleukin-6/*metabolism
MH  - Mammary Glands, Animal/*metabolism
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sialyltransferases/chemistry/*genetics/metabolism
MH  - Transcription, Genetic
MH  - beta-D-Galactoside alpha 2-6-Sialyltransferase
EDAT- 2009/07/21 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/07/21 09:00
PHST- 2009/07/21 09:00 [entrez]
PHST- 2009/07/21 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - cwp094 [pii]
AID - 10.1093/glycob/cwp094 [doi]
PST - ppublish
SO  - Glycobiology. 2009 Oct;19(10):1082-93. doi: 10.1093/glycob/cwp094. Epub 2009 Jul 
      17.