PMID- 1961736
OWN - NLM
STAT- MEDLINE
DCOM- 19920109
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 88
IP  - 23
DP  - 1991 Dec 1
TI  - Transcription factor requirement for multiple rounds of initiation by human RNA 
      polymerase II.
PG  - 10691-5
AB  - We have investigated conditions that allow multiple rounds of transcription 
      initiation from the adenovirus major late promoter in an in vitro system derived 
      from HeLa cell nuclear extracts. Templates containing guanine-free cassettes 
      provided a direct assay for discriminating between reinitiated transcripts and 
      transcripts generated by a first-round of transcription initiations. When 
      reactions were reconstituted with the previously characterized class II 
      transcription factors (TFIIA, TFIIB, TFIID, TFIIE/F), transcription by human RNA 
      polymerase II from the adenovirus major late promoter was essentially restricted 
      to a single round of initiations. Reinitiations at previously transcribed major 
      late templates required an additional activity, designated reinitiation 
      transcription factor (RTF). The RTF activity could be separated from the required 
      transcription initiation factors. Semipurified human RTF also promoted 
      transcription reinitiations at minimal promoters derived from the human c-myc, 
      histone H4, and heat shock 70-kDa protein genes, indicating that the same 
      reinitiation factor may be utilized by many, if not all, genes. The possible role 
      of RTF in regulating the transcription rate of various class II genes is 
      discussed.
FAU - Szentirmay, M N
AU  - Szentirmay MN
AD  - Department of Molecular Genetics, University of Texas, M. D. Anderson Cancer 
      Center, Houston 77030.
FAU - Sawadogo, M
AU  - Sawadogo M
LA  - eng
GR  - CA-09299/CA/NCI NIH HHS/United States
GR  - GM-38212/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factor TFIID)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics
MH  - HeLa Cells
MH  - Humans
MH  - Kinetics
MH  - *Promoter Regions, Genetic
MH  - RNA Polymerase II/*metabolism
MH  - Recombinant Proteins/isolation & purification/metabolism
MH  - Templates, Genetic
MH  - Transcription Factor TFIID
MH  - Transcription Factors/genetics/isolation & purification/*metabolism
MH  - *Transcription, Genetic
PMC - PMC52996
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
PMCR- 1992/06/01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
PHST- 1992/06/01 00:00 [pmc-release]
AID - 10.1073/pnas.88.23.10691 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10691-5. doi: 
      10.1073/pnas.88.23.10691.