PMID- 19619194
OWN - NLM
STAT- MEDLINE
DCOM- 20100618
LR  - 20220311
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Linking)
VI  - 16
IP  - 1
DP  - 2010 Jan
TI  - Combination of beta-TCP and BMP-2 gene-modified bMSCs to heal critical size 
      mandibular defects in rats.
PG  - 46-54
LID - 10.1111/j.1601-0825.2009.01602.x [doi]
AB  - OBJECTIVE: To investigate the effects of mandibular defects repaired by a tissue 
      engineered bone complex with beta-tricalcium phosphate (beta-TCP) and bone 
      morphogenic protein-2 (BMP-2) gene-modified bone marrow stromal cells (bMSCs). 
      MATERIALS AND METHODS: bMSCs derived from Fisher 344 rats were cultured and 
      transduced with adenovirus AdBMP-2, AdEGFP gene in vitro. Osteogenic 
      differentiation of bMSCs was determined by alkaline phosphatase staining, von 
      Kossa assay and reverse transcription-polymerase chain reaction. Gene transduced 
      or untransduced bMSCs were seeded on beta-TCP scaffolds to repair mandibular full 
      thickness defects with a diameter of 5 mm. Eight weeks post-operation, X-ray 
      examination, micro-computerized tomography and histological and 
      histomorphological analysis were used to evaluate the bone healing effects. 
      RESULTS: Alkaline phosphatase staining and mineralized nodules formation were 
      more pronounced in AdBMP-2 group 14 days after gene transduction when compared 
      with that of AdEGFP or untransduced group. The mRNA expression of osteopontin and 
      osteocalcin also significantly increased 9 days after AdBMP-2 gene transduction. 
      Mandibular defects were successfully repaired with AdBMP-2-transduced 
      bMSCs/beta-TCP constructs. The percentage of new bone formation in AdBMP-2 group 
      was significantly higher than that of other control groups. CONCLUSIONS: Bone 
      morphogenic protein-2 regional gene therapy together with beta-TCP scaffold could 
      be used to promote mandibular repairing and bone regeneration.
FAU - Zhao, J
AU  - Zhao J
AD  - Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth 
      People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200011 
      Shanghai, China.
FAU - Hu, J
AU  - Hu J
FAU - Wang, S
AU  - Wang S
FAU - Sun, X
AU  - Sun X
FAU - Xia, L
AU  - Xia L
FAU - Zhang, X
AU  - Zhang X
FAU - Zhang, Z
AU  - Zhang Z
FAU - Jiang, X
AU  - Jiang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090708
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Recombinant Proteins)
RN  - 0 (beta-tricalcium phosphate)
RN  - 104982-03-8 (Osteocalcin)
RN  - 106441-73-0 (Osteopontin)
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Bone Morphogenetic Protein 2/genetics/*pharmacology
MH  - Bone Regeneration/*drug effects
MH  - Calcium Phosphates
MH  - Cells, Cultured
MH  - Genetic Therapy/*methods
MH  - Male
MH  - Mandible/surgery
MH  - Osteoblasts/metabolism
MH  - Osteocalcin/biosynthesis
MH  - Osteopontin/biosynthesis
MH  - Rats
MH  - Rats, Inbred F344
MH  - Recombinant Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stromal Cells/*transplantation
MH  - Tissue Scaffolds
MH  - Transduction, Genetic
MH  - X-Ray Microtomography
EDAT- 2009/07/22 09:00
MHDA- 2010/06/19 06:00
CRDT- 2009/07/22 09:00
PHST- 2009/07/22 09:00 [entrez]
PHST- 2009/07/22 09:00 [pubmed]
PHST- 2010/06/19 06:00 [medline]
AID - ODI1602 [pii]
AID - 10.1111/j.1601-0825.2009.01602.x [doi]
PST - ppublish
SO  - Oral Dis. 2010 Jan;16(1):46-54. doi: 10.1111/j.1601-0825.2009.01602.x. Epub 2009 
      Jul 8.