PMID- 19619859
OWN - NLM
STAT- MEDLINE
DCOM- 20090929
LR  - 20090721
IS  - 1347-4421 (Electronic)
IS  - 1347-4421 (Linking)
VI  - 108
IP  - 2
DP  - 2009 Aug
TI  - Rapid detection and identification of beer-spoilage lactic acid bacteria by 
      microcolony method.
PG  - 124-9
LID - 10.1016/j.jbiosc.2009.02.016 [doi]
AB  - We evaluated a microcolony method for the detection and identification of 
      beer-spoilage lactic acid bacteria (LAB). In this approach, bacterial cells were 
      trapped on a polycarbonate membrane filter and cultured on ABD medium, a medium 
      that allows highly specific detection of beer-spoilage LAB strains. After 
      short-time incubation, viable cells forming microcolonies were stained with 
      carboxyfluorescein diacetate (CFDA) and counted with muFinder Inspection System. 
      In our study, we first investigated the growth behavior of various beer-spoilage 
      LAB by traditional culture method, and Lactobacillus lindneri and several L. 
      paracollinoides strains were selected as slow growers on ABD medium. Then the 
      detection speeds were evaluated by microcolony method, using these slowly growing 
      strains. As a result, all of the slowly growing beer-spoilage LAB strains were 
      detected within 3 days of incubation. The specificity of this method was found to 
      be exceptionally high and even discriminated intra-species differences in 
      beer-spoilage ability of LAB strains upon detection. These results indicate that 
      our microcolony approach allows rapid and specific detection of beer-spoilage LAB 
      strains with inexpensive CFDA staining. For further confirmation of species 
      status of detected strains, subsequent treatment with species-specific 
      fluorescence in situ hybridization (FISH) probes was shown as effective for 
      identifying the CFDA-detected microcolonies to the species level. In addition, no 
      false-positive results arising from noise signals were recognized for CFDA 
      staining and FISH methods. Taken together, the developed microcolony method was 
      demonstrated as a rapid and highly specific countermeasure against beer-spoilage 
      LAB, and compared favorably with the conventional culture methods.
FAU - Asano, Shizuka
AU  - Asano S
AD  - Research Laboratories of Brewing Technology, Asahi Breweries Ltd., 1-21 Midori 
      1-Chome, Moriya-shi, Ibaraki 302-0106, Japan.
FAU - Iijima, Kazumaru
AU  - Iijima K
FAU - Suzuki, Koji
AU  - Suzuki K
FAU - Motoyama, Yasuo
AU  - Motoyama Y
FAU - Ogata, Tomoo
AU  - Ogata T
FAU - Kitagawa, Yasushi
AU  - Kitagawa Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
SB  - IM
MH  - Beer/*microbiology
MH  - Colony Count, Microbial/methods
MH  - *Food Microbiology
MH  - Lactobacillus/growth & development/*isolation & purification
EDAT- 2009/07/22 09:00
MHDA- 2009/09/30 06:00
CRDT- 2009/07/22 09:00
PHST- 2009/01/13 00:00 [received]
PHST- 2009/02/23 00:00 [revised]
PHST- 2009/02/28 00:00 [accepted]
PHST- 2009/07/22 09:00 [entrez]
PHST- 2009/07/22 09:00 [pubmed]
PHST- 2009/09/30 06:00 [medline]
AID - S1389-1723(09)00136-4 [pii]
AID - 10.1016/j.jbiosc.2009.02.016 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2009 Aug;108(2):124-9. doi: 10.1016/j.jbiosc.2009.02.016.