PMID- 19622078 OWN - NLM STAT- MEDLINE DCOM- 20110118 LR - 20141120 IS - 1469-0691 (Electronic) IS - 1198-743X (Linking) VI - 16 IP - 6 DP - 2010 Jun TI - High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asuncion, Paraguay. PG - 624-7 LID - 10.1111/j.1469-0691.2009.02898.x [doi] AB - Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asuncion, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn1546 variants were found, including a new lineage containing an ISEfa5 insertion in an existing IS1251 element. FAU - Khan, M A AU - Khan MA AD - Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands. FAU - Northwood, J B AU - Northwood JB FAU - Loor, R G J AU - Loor RG FAU - Tholen, A T R AU - Tholen AT FAU - Riera, E AU - Riera E FAU - Falcon, M AU - Falcon M CN - Paraguayan Antimicrobial Network, FAU - van Belkum, A AU - van Belkum A FAU - van Westreenen, M AU - van Westreenen M FAU - Hays, J P AU - Hays JP LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090720 PL - England TA - Clin Microbiol Infect JT - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JID - 9516420 RN - 0 (Bacterial Proteins) RN - 0 (DNA Transposable Elements) RN - 0 (DNA, Bacterial) RN - 0 (VanA ligase, Bacteria) RN - EC 6.1.- (Carbon-Oxygen Ligases) SB - IM MH - Bacterial Proteins/genetics MH - Bacterial Typing Techniques MH - Carbon-Oxygen Ligases/genetics MH - Cluster Analysis MH - Cross Infection/*epidemiology/*microbiology MH - DNA Fingerprinting MH - DNA Transposable Elements MH - DNA, Bacterial/genetics MH - Electrophoresis, Gel, Pulsed-Field MH - Enterococcus faecium/*drug effects/isolation & purification MH - Genotype MH - Gram-Positive Bacterial Infections/*epidemiology/*microbiology MH - Hospitals MH - Humans MH - Paraguay/epidemiology MH - Prevalence MH - Sequence Analysis, DNA MH - *Vancomycin Resistance EDAT- 2009/07/23 09:00 MHDA- 2011/01/19 06:00 CRDT- 2009/07/23 09:00 PHST- 2009/07/23 09:00 [entrez] PHST- 2009/07/23 09:00 [pubmed] PHST- 2011/01/19 06:00 [medline] AID - S1198-743X(14)61700-6 [pii] AID - 10.1111/j.1469-0691.2009.02898.x [doi] PST - ppublish SO - Clin Microbiol Infect. 2010 Jun;16(6):624-7. doi: 10.1111/j.1469-0691.2009.02898.x. Epub 2009 Jul 20.