PMID- 19623584
OWN - NLM
STAT- MEDLINE
DCOM- 20091211
LR  - 20181201
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 108
IP  - 2
DP  - 2009 Oct 1
TI  - In vitro labeling of human umbilical cord mesenchymal stem cells with 
      superparamagnetic iron oxide nanoparticles.
PG  - 529-35
LID - 10.1002/jcb.22283 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUC-MSCs) transplantation has been 
      shown to promote regeneration and neuroprotection in central nervous system (CNS) 
      injuries and neurodegenerative diseases. To develop this approach into a clinical 
      setting it is important to be able to follow the fates of transplanted cells by 
      noninvasive imaging. Neural precursor cells and hematopoietic stem cells can be 
      efficiently labeled by superparamagnetic iron oxide (SPIO) nanoparticle. The 
      purpose of our study was to prospectively evaluate the influence of SPIO on 
      hUC-MSCs and the feasibility of tracking for hUC-MSCs by noninvasive imaging. In 
      vitro studies demonstrated that magnetic resonance imaging (MRI) can efficiently 
      detect low numbers of SPIO-labeled hUC-MSCs and that the intensity of the signal 
      was proportional to the number of labeled cells. After transplantation into focal 
      areas in adult rat spinal cord transplanted SPIO-labeled hUC-MSCs produced a 
      hypointense signal using T2-weighted MRI in rats that persisted for up to 2 
      weeks. This study demonstrated the feasibility of noninvasive imaging of 
      transplanted hUC-MSCs.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Hu, Sheng-Li
AU  - Hu SL
AD  - Department of Neurosurgery, Southwest Hospital, The Third Military Medical 
      University, Chongqing 400038, PR China.
FAU - Zhang, Jiu-Quan
AU  - Zhang JQ
FAU - Hu, Xiang
AU  - Hu X
FAU - Hu, Rong
AU  - Hu R
FAU - Luo, Hai-Shui
AU  - Luo HS
FAU - Li, Fei
AU  - Li F
FAU - Xia, Yong-Zhi
AU  - Xia YZ
FAU - Li, Jiang-Tao
AU  - Li JT
FAU - Lin, Jiang-Kai
AU  - Lin JK
FAU - Zhu, Gang
AU  - Zhu G
FAU - Feng, Hua
AU  - Feng H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antigens, CD)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Cell Survival
MH  - Female
MH  - Ferrosoferric Oxide/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Injections, Spinal
MH  - Laminectomy
MH  - Magnetic Resonance Imaging
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley/surgery
MH  - Regression Analysis
MH  - Spinal Cord/cytology/surgery
MH  - Time Factors
MH  - Umbilical Cord/*cytology
EDAT- 2009/07/23 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/07/23 09:00
PHST- 2009/07/23 09:00 [entrez]
PHST- 2009/07/23 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1002/jcb.22283 [doi]
PST - ppublish
SO  - J Cell Biochem. 2009 Oct 1;108(2):529-35. doi: 10.1002/jcb.22283.