PMID- 19624130
OWN - NLM
STAT- MEDLINE
DCOM- 20100715
LR  - 20191210
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 57
IP  - 16
DP  - 2009 Aug 26
TI  - Pressurized liquid extraction of soil microbial phospholipid and neutral lipid 
      fatty acids.
PG  - 7171-7
LID - 10.1021/jf901257n [doi]
AB  - Soil microbial lipid biomarkers are indicators of viable microbial biomass and 
      community structure. Pressurized liquid extraction (PLE) of soil phospholipid 
      fatty acids (PLFA) and neutral lipid fatty acids (NLFA) was compared to a 
      conventional extraction method in four soils with differing physical and chemical 
      properties. PLE efficiency was greater than that of the conventional method for 
      about half of the saturated PLFA and for selected other Gram-positive (i16:0) and 
      Gram-negative bacteria (18:1omega7c) PLFA, fungal PLFA (18:2omega6,9c), and 
      eukaryotic NLFA from a coarse-textured soil. Lipids extracted by the two methods 
      did not indicate a significant difference in microbial community structure data. 
      Principle component analysis revealed that PLFA clustered by location, with data 
      indicating that the group of microbes contributing the greatest weight differed 
      among soils. Overall, the PLE method proved to be more efficient at extracting 
      soilborne microbial lipids while not altering microbial community information. 
      These advantages indicate the PLE method is robust and well-suited to soil 
      microbial ecology research.
FAU - White, Paul M
AU  - White PM
AD  - Southeast Watershed Research Unit, Pesticide Residue Laboratory, Agricultural 
      Research Service, U.S. Department of Agriculture, Tifton, Georgia 31793, USA. 
      Paul.White@ars.usda.gov
FAU - Potter, Thomas L
AU  - Potter TL
FAU - Strickland, Timothy C
AU  - Strickland TC
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Fatty Acids)
RN  - 0 (Phospholipids)
SB  - IM
MH  - Bacteria/chemistry
MH  - Chemical Fractionation/*methods
MH  - Eukaryota/chemistry
MH  - Fatty Acids/analysis/*isolation & purification
MH  - Fungi/chemistry
MH  - Phospholipids/analysis/*isolation & purification
MH  - *Soil Microbiology
EDAT- 2009/07/25 09:00
MHDA- 2010/07/16 06:00
CRDT- 2009/07/24 09:00
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
AID - 10.1021/jf901257n [doi]
PST - ppublish
SO  - J Agric Food Chem. 2009 Aug 26;57(16):7171-7. doi: 10.1021/jf901257n.