PMID- 19624559
OWN - NLM
STAT- MEDLINE
DCOM- 20100120
LR  - 20101118
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 64
IP  - 11
DP  - 2009 Nov
TI  - IgE cross-reactivity between Ascaris and domestic mite allergens: the role of 
      tropomyosin and the nematode polyprotein ABA-1.
PG  - 1635-43
LID - 10.1111/j.1398-9995.2009.02084.x [doi]
AB  - BACKGROUND: Analysis of cross-reactivity between the nematode Ascaris ssp. and 
      dust mites, two important allergen sources in the tropics, will contribute in 
      understanding their influence on asthma and atopy. The objective of this study 
      was to investigate immunoglobulin E (IgE) cross-reactivity between Ascaris and 
      two domestic mites in the tropics. METHODS: Sera from 24 asthmatic patients were 
      used in ELISA and immunoblotting IgE-binding inhibition assays using Ascaris, 
      Blomia tropicalis and Dermatophagoides pteronyssinus extracts and the 
      recombinants Blo t 10, ABA-1 and Blo t 13 as competitors. Identification of 
      Ascaris allergens was confirmed by mass spectrometry (LC-MS/MS). RESULTS: We 
      detected at least 12 human IgE-binding components in Ascaris extract. Blomia 
      tropicalis and D. pteronyssinus inhibited 83.3% and 79% of IgE-binding to 
      Ascaris, while Ascaris inhibited 58.3% and 79.3% to B. tropicalis and D. 
      pteronyssinus respectively. Mite tropomyosin inhibited 85% of IgE-binding to 
      Ascaris. Affinity-purified human IgE to rBlo t 10 identified an allergen of 40 
      kDa in Ascaris extract, further confirmed as tropomyosin by LC-MS/MS. We found no 
      evidence of IgE cross-reactivity between rABA-1 and any allergen component in 
      mite extracts, including rBlo t 13. CONCLUSIONS: There is cross-reactivity 
      between Ascaris and mites, determined by several allergens including tropomyosin 
      and glutathione-S-transferase. In addition to its potential impact on asthma 
      pathogenesis, Ascaris infection and mite allergy diagnosis relying on the 
      determination of specific IgE could be affected by this cross-reactivity. ABA-1 
      has no cross-reactive counterpart in mite extracts, suggesting its usefulness as 
      a more specific marker of Ascaris infection.
FAU - Acevedo, N
AU  - Acevedo N
AD  - Institute for Immunological Research, University of Cartagena, Cartagena, 
      Colombia.
FAU - Sanchez, J
AU  - Sanchez J
FAU - Erler, A
AU  - Erler A
FAU - Mercado, D
AU  - Mercado D
FAU - Briza, P
AU  - Briza P
FAU - Kennedy, M
AU  - Kennedy M
FAU - Fernandez, A
AU  - Fernandez A
FAU - Gutierrez, M
AU  - Gutierrez M
FAU - Chua, K Y
AU  - Chua KY
FAU - Cheong, N
AU  - Cheong N
FAU - Jimenez, S
AU  - Jimenez S
FAU - Puerta, L
AU  - Puerta L
FAU - Caraballo, L
AU  - Caraballo L
LA  - eng
GR  - Medical Research Council/United Kingdom
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090717
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (ABA-1 protein, Ascaris suum)
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Antigens, Plant)
RN  - 0 (Helminth Proteins)
RN  - 0 (Tropomyosin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/*immunology
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Antigens, Plant
MH  - Ascaris/*immunology
MH  - *Asthma/immunology/physiopathology
MH  - Child
MH  - Cross Reactions
MH  - Female
MH  - Glutathione Transferase/immunology
MH  - Helminth Proteins/immunology
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology/physiopathology
MH  - *Immunoglobulin E/immunology
MH  - Male
MH  - Middle Aged
MH  - Mites/*immunology
MH  - Tropomyosin/*immunology
MH  - Young Adult
EDAT- 2009/07/25 09:00
MHDA- 2010/01/21 06:00
CRDT- 2009/07/24 09:00
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2010/01/21 06:00 [medline]
AID - ALL2084 [pii]
AID - 10.1111/j.1398-9995.2009.02084.x [doi]
PST - ppublish
SO  - Allergy. 2009 Nov;64(11):1635-43. doi: 10.1111/j.1398-9995.2009.02084.x. Epub 
      2009 Jul 17.