PMID- 19624613
OWN - NLM
STAT- MEDLINE
DCOM- 20091222
LR  - 20221207
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 74
IP  - 4
DP  - 2009 Oct
TI  - An HLA-DR11/DQ3 haplotype with a DRB1*0301 sequence motif in the third 
      hypervariable region of the HLA-DR beta-1 chain: molecular and serological 
      analysis of its generation in a European Caucasian family.
PG  - 330-5
LID - 10.1111/j.1399-0039.2009.01315.x [doi]
AB  - The formation of a new human leukocyte antigen (HLA)-DRB1 allele (DRB1*0340) has 
      been detected during the routine testing of a European Caucasian blood and 
      potential stem cell donor and his family. HLA typing of the donor with two 
      polymerase chain reaction - sequence specific oligonucleotides (PCR-SSO) systems 
      yielded inconclusive results. HLA typing of the family members including 
      sequence-based typing of DRB1 in both directions after haplotype-specific 
      amplification showed that the allele had most likely formed by a double crossover 
      event in exon 2 of the DRB1 gene. The HLA haplotype containing the new allele was 
      most probably derived from the father, who was typed as HLA-DRB1*0301,*1101 and 
      DRB3*0101,*0202. The comparison of the sequences of the paternal DRB1 and DRB3 
      alleles with the exon 2 sequence of the DRB1*0340 showed that it had most likely 
      formed through an uptake of at least the sequence part codons 58-77 of DRB1*0301 
      (donor) by DRB1*1101 (acceptor). We suppose that the recombination sites are 
      located in the sequences from codons 38-57 and codons 78-88. At the protein 
      level, more than 50% of the alpha-helical structure of the DRB1*1101 chain is 
      replaced by a DRB1*0301-derived sequence with the exchange of several amino 
      acids. Serological typing of the allele showed HLA-DR3. However, one monoclonal 
      anti-DR11 of five DR11-reactive antibodies reacted positive, which might indicate 
      residual immunogenic epitopes of DRB1*1101. HLA alleles that are most similar to 
      HLA-DRB1*0340 are DRB1*030501, *0317, *0329 and *1107 with at least four amino 
      acid differences in exon 2. In conclusion, HLA-DRB1*0340 is a new allele with 
      unique properties compared with other known HLA-DRB alleles with regard to 
      antigenicity, T-cell receptor-binding and peptide-binding possibilities.
FAU - Kelsch, R
AU  - Kelsch R
AD  - Institute of Transfusion Medicine and Transplantation Immunology, University 
      Clinics of Muenster, Muenster, Germany. kelsch@uni-muenster.de
FAU - Binder, T M C
AU  - Binder TM
FAU - Elsner, H A
AU  - Elsner HA
FAU - Eiermann, T H
AU  - Eiermann TH
FAU - Sibrowski, W
AU  - Sibrowski W
LA  - eng
PT  - Journal Article
DEP - 20090715
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Complementarity Determining Regions)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ3 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DR Serological Subtypes)
RN  - 0 (HLA-DR11 antigen)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*03:01 antigen)
SB  - IM
MH  - Base Sequence
MH  - Complementarity Determining Regions/*genetics
MH  - Female
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DR Antigens/*blood/*genetics
MH  - HLA-DR Serological Subtypes
MH  - HLA-DRB1 Chains
MH  - Haplotypes/*genetics
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Pedigree
MH  - Phylogeny
MH  - Sequence Homology, Nucleic Acid
MH  - White People/*genetics
EDAT- 2009/07/25 09:00
MHDA- 2009/12/23 06:00
CRDT- 2009/07/24 09:00
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2009/12/23 06:00 [medline]
AID - TAN1315 [pii]
AID - 10.1111/j.1399-0039.2009.01315.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2009 Oct;74(4):330-5. doi: 10.1111/j.1399-0039.2009.01315.x. 
      Epub 2009 Jul 15.