PMID- 19624615
OWN - NLM
STAT- MEDLINE
DCOM- 20091222
LR  - 20090924
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 74
IP  - 4
DP  - 2009 Oct
TI  - New tools to monitor the impact of viral infection on the alloreactive T-cell 
      repertoire.
PG  - 290-7
LID - 10.1111/j.1399-0039.2009.01311.x [doi]
AB  - Accumulating evidence suggests that alloreactive memory T-cells may be generated 
      as a result of viral infection. So far, a suitable tool to define the individual 
      human leukocyte antigen (HLA) cross-reactivity of virus-specific memory T-cells 
      is not available. We therefore aimed to develop a novel system for the detection 
      of cross-reactive alloresponses using single HLA antigen expressing cell lines 
      (SALs) as stimulator. Herein, we generated Epstein-Barr Virus (EBV) EBNA3A 
      specific CD8 memory T-cell clones (HLA-B*0801/FLRGRAYGL peptide restricted) and 
      assayed for alloreactivity against a panel of SALs using interferon-gamma Elispot 
      as readout. Generation of the T-cell clones was performed by single cell sorting 
      based on staining with viral peptide/major histocompatibility complex-specific 
      tetramer. Monoclonality of the T-cell clones was confirmed by T-cell receptor 
      (TCR) polymerase chain reaction analysis. First, we confirmed the previously 
      described alloreactivity of the EBV EBNA3A-specific T-cell clones against 
      SAL-expressing HLA-B*4402. Further screening against the entire panel of SALs 
      also showed additional cross-reactivity against SAL-expressing HLA-B*5501. 
      Functionality of the cross-reactive T-cell clones was confirmed by chromium 
      release assay using phytohemagglutinin blasts as targets. SALs are an effective 
      tool to detect cross-reactivity of viral-specific CD8 memory T-cell clones 
      against individual class I HLA molecules. This technique may have important 
      implications for donor selection and monitoring of transplant recipients.
FAU - D'Orsogna, L J
AU  - D'Orsogna LJ
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Centre, Leiden, The Netherlands. l.j.a.d_orsogna@lumc.nl
FAU - Amir, A L
AU  - Amir AL
FAU - Zoet, Y M
AU  - Zoet YM
FAU - van der Meer-Prins, P M W
AU  - van der Meer-Prins PM
FAU - van der Slik, A R
AU  - van der Slik AR
FAU - Kester, M G D
AU  - Kester MG
FAU - Heemskerk, M H M
AU  - Heemskerk MH
FAU - Doxiadis, I I N
AU  - Doxiadis II
FAU - Roelen, D L
AU  - Roelen DL
FAU - Claas, F H J
AU  - Claas FH
LA  - eng
PT  - Journal Article
DEP - 20090715
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antigens, Surface)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (HLA Antigens)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Antigen Presentation
MH  - Antigens, Surface/immunology
MH  - Antigens, Viral/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cross Reactions
MH  - DNA Primers/chemistry
MH  - Epstein-Barr Virus Infections/genetics/*immunology/virology
MH  - HLA Antigens/*immunology/metabolism
MH  - Herpesvirus 4, Human/immunology/*pathogenicity
MH  - Humans
MH  - *Immunologic Memory
MH  - Peptide Fragments/immunology
MH  - Receptors, Antigen, T-Cell/immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2009/07/25 09:00
MHDA- 2009/12/23 06:00
CRDT- 2009/07/24 09:00
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2009/12/23 06:00 [medline]
AID - TAN1311 [pii]
AID - 10.1111/j.1399-0039.2009.01311.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2009 Oct;74(4):290-7. doi: 10.1111/j.1399-0039.2009.01311.x. 
      Epub 2009 Jul 15.