PMID- 19625464
OWN - NLM
STAT- MEDLINE
DCOM- 20091030
LR  - 20211028
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 90
IP  - Pt 11
DP  - 2009 Nov
TI  - Microarray-based determination of the lytic cascade of human herpesvirus 6B.
PG  - 2581-2591
LID - 10.1099/vir.0.012815-0 [doi]
AB  - The lytic gene expression of several members of the human herpesvirus family has 
      been profiled by using gene-expression microarrays; however, the lytic cascade of 
      roseoloviruses has not been studied in similar depth. Based on the complete DNA 
      genome sequences of human herpesvirus 6 variant A (HHV-6A) and variant B 
      (HHV-6B), we constructed a cDNA microarray containing DNA probes to their 
      predicted open reading frames, plus 914 human genes. Gene-expression profiling of 
      HHV-6B strain Z29 in SupT1 cells over a 60 h time-course post-infection, together 
      with kinetic classification of the HHV-6B genes in the presence of either 
      cycloheximide or phosphonoacetic acid, allowed the placement of HHV-6B genes into 
      defined kinetic classes. Eighty-nine HHV-6B genes were divided into four 
      different expression kinetic classes: eight immediate-early, 44 early, 33 late 
      and four biphasic. Clustering of genes with similar expression profiles implied a 
      shared function, thus revealing possible roles of previously uncharacterized 
      HHV-6B genes.
FAU - Tsao, Edward H
AU  - Tsao EH
AD  - Department of Infection, Division of Infection and Immunity, Royal Free and 
      University College Medical School of UCL, London, UK.
FAU - Kellam, Paul
AU  - Kellam P
AD  - Virus Genomics Team, Wellcome Trust Sanger Institute, Cambridge, UK.
AD  - Department of Infection, Division of Infection and Immunity, Royal Free and 
      University College Medical School of UCL, London, UK.
FAU - Sin, Cheryl S Y
AU  - Sin CSY
AD  - Department of Infection, Division of Infection and Immunity, Royal Free and 
      University College Medical School of UCL, London, UK.
FAU - Rasaiyaah, Jane
AU  - Rasaiyaah J
AD  - Department of Infection, Division of Infection and Immunity, Royal Free and 
      University College Medical School of UCL, London, UK.
FAU - Griffiths, Paul D
AU  - Griffiths PD
AD  - Department of Infection, Division of Infection and Immunity, Royal Free and 
      University College Medical School of UCL, London, UK.
FAU - Clark, Duncan A
AU  - Clark DA
AD  - Department of Infection, Division of Infection and Immunity, Royal Free and 
      University College Medical School of UCL, London, UK.
LA  - eng
GR  - EGM17738/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090722
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antiviral Agents)
RN  - 98600C0908 (Cycloheximide)
RN  - N919E46723 (Phosphonoacetic Acid)
SB  - IM
MH  - Antiviral Agents/pharmacology
MH  - Cell Line
MH  - Cycloheximide/pharmacology
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Viral
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - *Oligonucleotide Array Sequence Analysis/methods
MH  - Phosphonoacetic Acid/pharmacology
MH  - *Virus Replication
EDAT- 2009/07/25 09:00
MHDA- 2009/10/31 06:00
CRDT- 2009/07/24 09:00
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2009/10/31 06:00 [medline]
AID - 10.1099/vir.0.012815-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2009 Nov;90(Pt 11):2581-2591. doi: 10.1099/vir.0.012815-0. Epub 2009 
      Jul 22.