PMID- 19625644
OWN - NLM
STAT- MEDLINE
DCOM- 20090909
LR  - 20211028
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 183
IP  - 4
DP  - 2009 Aug 15
TI  - TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived 
      dendritic cells fail to induce Th17 polarization of naive T cells but do 
      stimulate Th1 and Th17 memory responses.
PG  - 2242-51
LID - 10.4049/jimmunol.0900474 [doi]
AB  - Dendritic cells (DCs) are important in linking innate and adaptive immune 
      responses by priming and polarizing naive CD4(+) Th cells, but little is known 
      about the effect of different human DC subsets on Th cells, particularly Th17 
      cells. We have investigated the ability of TLR-stimulated human Langerhans cells 
      (LC), dermal DCs (dDC), and monocyte-derived DCs (moDC) to affect naive and 
      memory Th17 and Th1 responses. MoDCs stimulated greater memory T cell 
      proliferation while LCs and dDCs more potently stimulated naive T cell 
      proliferation, indicating functionally distinct subsets of DCs. TLR stimulation 
      of all three DC types was unable to induce Th17 polarization from naive T cell 
      precursors, despite inducing Th1 polarization. Dectin stimulation of DCs in IMDM 
      was however able to produce Th17 cells. TLR-stimulated DCs were capable of 
      inducing IL-17A and IFN-gamma production from memory T cells, although the 
      mechanism used by each DC subset differed. MoDCs partially mediated this effect 
      on memory Th1 and Th17 cells by the production of soluble factors, which 
      correlated with their ability to secrete IL-12p70 and IL-23. In contrast, LCs and 
      dDCs were able to elicit a similar memory response to moDCs, but in a contact 
      dependent manner. Additionally, the influence of microbial stimulation was 
      demonstrated with TLR3 and TLR7/8 agonists inducing a Th1 response, whereas TLR2 
      or dectin stimulation of moDCs enhanced the IL-17 response. This study emphasizes 
      the differences between human DC subsets and demonstrates that both the DC subset 
      and the microbial stimulus influence the Th cell response.
FAU - Duraisingham, Sai Suda
AU  - Duraisingham SS
AD  - Department of Immunology, Faculty of Medicine, Imperial College, Chelsea and 
      Westminster Hospital, London, United Kingdom.
FAU - Hornig, Julia
AU  - Hornig J
FAU - Gotch, Frances
AU  - Gotch F
FAU - Patterson, Steven
AU  - Patterson S
LA  - eng
GR  - G0100482/MRC_/Medical Research Council/United Kingdom
GR  - G0501957/MRC_/Medical Research Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090722
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD34)
RN  - 0 (Interleukin-17)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Adult
MH  - Antigens, CD34/biosynthesis
MH  - Cell Differentiation/immunology
MH  - Cell Polarity/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/classification/*immunology/metabolism
MH  - Female
MH  - Fetal Blood/cytology/immunology/metabolism
MH  - Humans
MH  - *Immunologic Memory
MH  - Interleukin-17/*biosynthesis/metabolism
MH  - Lymphocyte Activation/*immunology
MH  - Monocytes/immunology/metabolism
MH  - Receptors, Antigen, T-Cell/agonists/biosynthesis/*physiology
MH  - Resting Phase, Cell Cycle/immunology
MH  - Skin/cytology/*immunology
MH  - Stem Cells/*immunology/metabolism
MH  - T-Lymphocytes, Helper-Inducer/cytology/immunology
MH  - Th1 Cells/cytology/*immunology
MH  - Th2 Cells/cytology/immunology
EDAT- 2009/07/25 09:00
MHDA- 2009/09/10 06:00
CRDT- 2009/07/24 09:00
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2009/09/10 06:00 [medline]
AID - jimmunol.0900474 [pii]
AID - 10.4049/jimmunol.0900474 [doi]
PST - ppublish
SO  - J Immunol. 2009 Aug 15;183(4):2242-51. doi: 10.4049/jimmunol.0900474. Epub 2009 
      Jul 22.