PMID- 19626401
OWN - NLM
STAT- MEDLINE
DCOM- 20091008
LR  - 20211020
IS  - 1615-2573 (Electronic)
IS  - 0910-8327 (Linking)
VI  - 24
IP  - 4
DP  - 2009 Jul
TI  - Interleukin-2 enhances the cytotoxic activity of circulating natural killer cells 
      in patients with chronic heart failure.
PG  - 283-6
LID - 10.1007/s00380-008-1123-0 [doi]
AB  - Our objective was to investigate the effect of interleukin-2 (IL-2) on the 
      cytotoxic activity of natural killer (NK) cells in patients with chronic heart 
      failure (CHF). Natural killer cells were isolated from 48 patients with CHF and 
      30 healthy subjects. The cytotoxic activities of the NK cells were assessed with 
      the thiazolyl blue tetrazolium bromide (MTT) approach. Interleukin-2 (20 ng/ml) 
      was added to the cell culture to stimulate the cytotoxicity of the NK cells. The 
      cell number in the New York Heart Association (NYHA) class II, III, and IV was 
      27.9 +/- 2.5, 21.2 +/- 2.7, and 16.8 +/- 2.6 cells/microl, respectively, which 
      was significantly lower than in the control group (31.2 +/- 3.6 cells/microl, all 
      P < 0.01). The cytotoxic activities in NYHA II, III, and IV groups were 28.6% +/- 
      3.2%, 16.0% +/- 2.2%, and 12.1% +/- 2.9%, respectively, which was lower than in 
      the control group (41.0% +/- 4.0%, all P < 0.01). Univariate analysis showed a 
      close correlation between the NK cell cytotoxicity and the left ventricular 
      ejection fraction (r = 0.949, P < 0.001). After in vitro treatment with IL-2, 
      there was a significant increase in the cytotoxic activity of the NK cells in the 
      control and the three heart failure groups (all P < 0.01). There is a significant 
      reduction in the number and the cytotoxic activity of circulating NK cells in 
      patients with CHF. The degree of NK cell deficiency is closely related to the 
      severity of left ventricular dysfunction. In vitro treatment with IL-2 improves 
      the cytotoxic activity of NK cell from the CHF patients.
FAU - Yao, Heng-Chen
AU  - Yao HC
AD  - Department of Cardiology, Liaocheng People's Hospital and Liaocheng Clinical 
      School of Taishan Medical College, Liaocheng City, Shandong Province, PR China.
FAU - Liu, Shu-Qin
AU  - Liu SQ
FAU - Yu, Ke
AU  - Yu K
FAU - Zhou, Min
AU  - Zhou M
FAU - Wang, Le-Xin
AU  - Wang LX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090722
PL  - Japan
TA  - Heart Vessels
JT  - Heart and vessels
JID - 8511258
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Chronic Disease
MH  - Coculture Techniques
MH  - *Cytotoxicity, Immunologic
MH  - Female
MH  - Heart Failure/complications/*immunology/physiopathology
MH  - Humans
MH  - Interleukin-2/*metabolism
MH  - K562 Cells
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - Severity of Illness Index
MH  - Stroke Volume
MH  - Ventricular Dysfunction, Left/*immunology/physiopathology
EDAT- 2009/07/25 09:00
MHDA- 2009/10/09 06:00
CRDT- 2009/07/24 09:00
PHST- 2008/06/10 00:00 [received]
PHST- 2008/10/09 00:00 [accepted]
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2009/10/09 06:00 [medline]
AID - 10.1007/s00380-008-1123-0 [doi]
PST - ppublish
SO  - Heart Vessels. 2009 Jul;24(4):283-6. doi: 10.1007/s00380-008-1123-0. Epub 2009 
      Jul 22.