PMID- 19626662
OWN - NLM
STAT- MEDLINE
DCOM- 20091211
LR  - 20171116
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 108
IP  - 2
DP  - 2009 Oct 1
TI  - Inhibition of phosphatidylcholine-specific phospholipase C prevents bone marrow 
      stromal cell senescence in vitro.
PG  - 519-28
LID - 10.1002/jcb.22282 [doi]
AB  - Bone marrow stromal cells (BMSCs) can proliferate in vitro and can be 
      transplanted for treating many kinds of diseases. However, BMSCs become senescent 
      with long-term culture, which inhibits their application. To understand the 
      mechanism underlying the senescence, we investigated the activity of 
      phosphatidylcholine-specific phospholipase C (PC-PLC) and levels of integrin 
      beta4, caveolin-1 and ROS with BMSC senescence. The activity of PC-PLC and levels 
      of integrin beta4, caveolin-1 and ROS increased greatly during cell senescence. 
      Selective inhibition of increased PC-PLC activity with D609 significantly 
      decreased the number of senescence-associated beta galactosidase positive cells 
      in BMSCs. Furthermore, D609 restored proliferation of BMSCs and their 
      differentiation into adipocytes. Moreover, D609 suppressed the elevated levels of 
      integrin beta4, caveolin-1 and ROS. The data suggest that PC-PLC is involved in 
      senescence of BMSCs, and its function is associated with integrin beta4, 
      caveolin-1 and ROS.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Sun, Chunhui
AU  - Sun C
AD  - Institute of Developmental Biology, School of Life Science, Shandong University, 
      Jinan 250100, China.
FAU - Wang, Nan
AU  - Wang N
FAU - Huang, Jie
AU  - Huang J
FAU - Xin, Jie
AU  - Xin J
FAU - Peng, Fen
AU  - Peng F
FAU - Ren, Yinshi
AU  - Ren Y
FAU - Zhang, Shangli
AU  - Zhang S
FAU - Miao, Junying
AU  - Miao J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Caveolin 1)
RN  - 0 (Integrin beta4)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adipogenesis
MH  - Animals
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Calcium/metabolism
MH  - Caveolin 1/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - *Cellular Senescence
MH  - Integrin beta4/metabolism
MH  - Male
MH  - Phosphatidylcholines/*metabolism
MH  - Protein Transport
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Stromal Cells/*metabolism/pathology
MH  - Substrate Specificity
MH  - Time Factors
MH  - Type C Phospholipases/*antagonists & inhibitors/metabolism
MH  - beta-Galactosidase/metabolism
EDAT- 2009/07/25 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/07/24 09:00
PHST- 2009/07/24 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1002/jcb.22282 [doi]
PST - ppublish
SO  - J Cell Biochem. 2009 Oct 1;108(2):519-28. doi: 10.1002/jcb.22282.