PMID- 19626848
OWN - NLM
STAT- MEDLINE
DCOM- 20101021
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 2
DP  - 2009 Mar
TI  - [Preparation of weak cation exchange monolithic column and its applications for 
      on-line determination of nifedipine in human plasma].
PG  - 197-200
AB  - A cation exchange monolithic column was prepared with methylacrylic acid (MAA) as 
      the functional monomer and ethylene dimethacrylate (EDMA) as the cross linker. 
      This column was applied to remove the matrix compounds and enrich the ionic 
      medicines in human plasma with water as the mobile phase. As a result, the human 
      plasma samples can be directly injected into chromatographic system. The 
      relationship between the mobile phase flow rate and back pressure was studied. 
      The results showed that the monolithic column had good performances in lower 
      pressure and higher permeability. In addition, the maximum adsorption of 
      nifedipine on this monolithic column was investigated. The on-line clean-up and 
      enrichment of samples were carried out using this column as the solid-phase 
      extraction material and the C18 column as the analytical column. The 
      chromatography was performed on a C18 reversed-phase high performance liquid 
      chromatographic column with ultraviolet detection at 235 nm. The mobile phase was 
      a mixture of methanol-water (70:30, v/v), and the flow rate was 1.0 mL/min. The 
      linear range of nifedipine in human plasma was 5.0-75.0 microg/L. The intra- and 
      inter-day relative standard deviations (RSDs) were both less than 5.0%. The limit 
      of detection (LOD) was 1 microg/L and the limit of quantification (LOQ) was 4 
      microg/L. In this method tedious pretreatment procedure is not necessary. It is a 
      fast, economical, reproducible and efficient method for assaying trace nifedipine 
      in human plasma.
FAU - Yang, Xinru
AU  - Yang X
AD  - College of Pharmacy, Hebei University, Hebei Province Key Laboratory of 
      Pharmaceutical Quality Control, Baodind 071002, China.
FAU - Yang, Gengliang
AU  - Yang G
FAU - Zhu, Tao
AU  - Zhu T
FAU - Feng, Xiaojuan
AU  - Feng X
FAU - Yang, Guanqun
AU  - Yang G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Acrylates)
RN  - 0 (Cations)
RN  - 0 (Hydroxylamines)
RN  - 0 (Methacrylates)
RN  - 0 (N,O-dimethacryloylhydroxylamine)
RN  - I9ZF7L6G2L (Nifedipine)
RN  - WC487PR91H (methyl acrylate)
SB  - IM
MH  - Acrylates/chemistry
MH  - Cations/chemistry
MH  - Chromatography, High Pressure Liquid/*instrumentation/methods
MH  - Chromatography, Ion Exchange/instrumentation/methods
MH  - Humans
MH  - Hydroxylamines/chemistry
MH  - Ion Exchange
MH  - Methacrylates/chemistry
MH  - Nifedipine/*blood
MH  - Solid Phase Extraction/*methods
EDAT- 2009/07/25 09:00
MHDA- 2010/10/22 06:00
CRDT- 2009/07/25 09:00
PHST- 2009/07/25 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2010/10/22 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 Mar;27(2):197-200.