PMID- 19628663
OWN - NLM
STAT- MEDLINE
DCOM- 20091123
LR  - 20211020
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 37
IP  - 17
DP  - 2009 Sep
TI  - YY1's role in DNA methylation of Peg3 and Xist.
PG  - 5656-64
LID - 10.1093/nar/gkp613 [doi]
AB  - Unusual clusters of YY1 binding sites are located within several differentially 
      methylated regions (DMRs), including Xist, Nespas and Peg3, which all become 
      methylated during oogenesis. In this study, we performed conditional YY1 
      knockdown (KD) to investigate YY1's roles in DNA methylation of these DMRs. 
      Reduced levels of YY1 during spermatogenesis did not cause any major change in 
      these DMRs although the same YY1 KD caused hypermethylation in these DMRs among a 
      subset of aged mice. However, YY1 KD during oogenesis resulted in the loss of DNA 
      methylation on Peg3 and Xist, but there were no changes on Nespas and H19. 
      Continued YY1 KD from oogenesis to the blastocyst stage caused further loss in 
      DNA methylation on Peg3. Consequently, high incidents of lethality were observed 
      among embryos that had experienced the reduced levels of YY1 protein. Overall, 
      the current study suggests that YY1 likely plays a role in the de novo DNA 
      methylation of the DMRs of Peg3 and Xist during oogenesis and also in the 
      maintenance of unmethylation status of these DMRs during spermatogenesis.
FAU - Kim, Jeong Do
AU  - Kim JD
AD  - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 
      70803, USA. jkim@lsu.edu
FAU - Kang, Keunsoo
AU  - Kang K
FAU - Kim, Joomyeong
AU  - Kim J
LA  - eng
GR  - R01 GM066225/GM/NIGMS NIH HHS/United States
GR  - R01GM66225/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090723
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Peg3 protein, mouse)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (XIST non-coding RNA)
RN  - 0 (YY1 Transcription Factor)
RN  - 0 (Yy1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Blastocyst/metabolism
MH  - *DNA Methylation
MH  - Female
MH  - Gene Knockdown Techniques
MH  - Germ Cells/growth & development
MH  - Kruppel-Like Transcription Factors/*genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Oogenesis/*genetics
MH  - RNA, Long Noncoding
MH  - RNA, Untranslated/*genetics/metabolism
MH  - Spermatogenesis/*genetics
MH  - YY1 Transcription Factor/genetics/*physiology
PMC - PMC2761279
EDAT- 2009/07/25 09:00
MHDA- 2009/12/16 06:00
PMCR- 2009/07/23
CRDT- 2009/07/25 09:00
PHST- 2009/07/25 09:00 [entrez]
PHST- 2009/07/25 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2009/07/23 00:00 [pmc-release]
AID - gkp613 [pii]
AID - 10.1093/nar/gkp613 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2009 Sep;37(17):5656-64. doi: 10.1093/nar/gkp613. Epub 2009 
      Jul 23.