PMID- 19630985
OWN - NLM
STAT- MEDLINE
DCOM- 20100121
LR  - 20211020
IS  - 1756-9966 (Electronic)
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 28
IP  - 1
DP  - 2009 Jul 25
TI  - Mutation analysis of Rad18 in human cancer cell lines and non small cell lung 
      cancer tissues.
PG  - 106
LID - 10.1186/1756-9966-28-106 [doi]
AB  - BACKGROUND: Genetic instability is known as a cause of oncogenesis. Though Rad18 
      is reported to function in a post replication mismatch repair system, the 
      relation between the status of Rad18 and human tumorigenesis has not been 
      described so far. METHODS: Mutation analysis of 34 human cancer cell lines and 32 
      non small cell lung cancer (NSCLC) tissues were performed by RT-PCR SSCP. 
      Expression level of Rad18 was measured by real time RT-PCR. Stable transfectant 
      was constructed for in vitro study. RESULTS: No mutation was found in both cancer 
      cell lines and NSCLC tissues. A single nucleotide polymorphism (SNP) at codon 302 
      was detected in 51.5% of the cell lines and 62.5% of NSCLC tissues. 
      Interestingly, Rad18 was homozygously deleted in a pulmonary adenocarcinoma cell 
      line PC3. Furthermore, there was no difference in the expression level of wild 
      type Rad18 and Rad18 with SNP. The growth, cell morphology, sensitivity to 
      anti-cancer drugs and in vitro DNA repair activity between wild type Rad18 and 
      Rad18 with SNP revealed to have no difference in vitro. CONCLUSION: Though the 
      frequency of SNP was tended to be higher in NSCLC patients than healthy 
      volunteers (57.7%), as the difference was not significant, we have concluded that 
      there is no relation between Rad18 SNP and lung cancer development.
FAU - Nakamura, Tadahiko
AU  - Nakamura T
AD  - Department of Gatroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto 860-8556, Japan. tadahiko@mars.dti.ne.jp
FAU - Ishikawa, Shinji
AU  - Ishikawa S
FAU - Koga, Yoshikatsu
AU  - Koga Y
FAU - Nagai, Youhei
AU  - Nagai Y
FAU - Imamura, Yu
AU  - Imamura Y
FAU - Ikeda, Kouei
AU  - Ikeda K
FAU - Mori, Takeshi
AU  - Mori T
FAU - Nomori, Hiroaki
AU  - Nomori H
FAU - Baba, Hideo
AU  - Baba H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090725
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RAD18 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Cell Line, Tumor
MH  - *DNA Mutational Analysis
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lung Neoplasms/*genetics/pathology
MH  - Neoplasms/genetics/pathology
MH  - Polymorphism, Single-Stranded Conformational
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ubiquitin-Protein Ligases
PMC - PMC2723085
EDAT- 2009/07/28 09:00
MHDA- 2010/01/22 06:00
PMCR- 2009/07/25
CRDT- 2009/07/28 09:00
PHST- 2009/04/19 00:00 [received]
PHST- 2009/07/25 00:00 [accepted]
PHST- 2009/07/28 09:00 [entrez]
PHST- 2009/07/28 09:00 [pubmed]
PHST- 2010/01/22 06:00 [medline]
PHST- 2009/07/25 00:00 [pmc-release]
AID - 1756-9966-28-106 [pii]
AID - 10.1186/1756-9966-28-106 [doi]
PST - epublish
SO  - J Exp Clin Cancer Res. 2009 Jul 25;28(1):106. doi: 10.1186/1756-9966-28-106.