PMID- 19631699
OWN - NLM
STAT- MEDLINE
DCOM- 20091109
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1793
IP  - 10
DP  - 2009 Oct
TI  - Store-operated Ca2+ entry is sensitive to the extracellular Ca2+ concentration 
      through plasma membrane STIM1.
PG  - 1614-22
LID - 10.1016/j.bbamcr.2009.07.003 [doi]
AB  - Store-operated Ca(2+) entry (SOCE) is a major mechanism for Ca(2+) influx in 
      platelets and other cells activated by a reduction in Ca(2+) concentration in the 
      intracellular stores. SOCE has been reported to be regulated by extracellular 
      Ca(2+), although the underlying mechanism remains unclear. Here we have examined 
      the involvement of plasma membrane-located STIM1 (PM-STIM1) in the regulation of 
      SOCE by extracellular Ca(2+). Treatment of platelets with the SERCA inhibitor 
      thapsigargin (TG) induced Mn(2+) entry, which was inhibited by extracellular 
      Ca(2+) in a concentration-dependent manner. Incubation of platelets with a 
      specific antibody, which recognizes the extracellular amino acid sequence 25-139 
      of PM-STIM1 that contains the Ca(2+)-binding domain, prevented the inactivation 
      of Ca(2+) entry induced by extracellular Ca(2+). TG induced translocation of 
      STIM1 to the plasma membrane (PM), an event that was found to be 
      Ca(2+)-dependent. In addition, TG stimulated association of PM-STIM1 with Orai1, 
      an event that was not prevented by stabilization of the membrane cytoskeleton 
      using jasplakinolide. These findings suggest that PM-STIM1 is important for the 
      inactivation of SOCE by extracellular Ca(2+), an event that is likely to be 
      mediated by interaction with Orai1.
FAU - Jardin, Isaac
AU  - Jardin I
AD  - Department of Physiology (Cellular Physiology Research Group), University of 
      Extremadura, 10071 Caceres, Spain.
FAU - Lopez, Jose J
AU  - Lopez JJ
FAU - Redondo, Pedro C
AU  - Redondo PC
FAU - Salido, Gines M
AU  - Salido GM
FAU - Rosado, Juan A
AU  - Rosado JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090723
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Calcium Channels)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (ORAI1 Protein)
RN  - 0 (ORAI1 protein, human)
RN  - 0 (STIM1 protein, human)
RN  - 0 (Stromal Interaction Molecule 1)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Blood Platelets/drug effects/*metabolism
MH  - Calcium/metabolism/pharmacology
MH  - Calcium Channels/blood
MH  - Calcium Signaling/drug effects/*physiology
MH  - Cell Membrane/drug effects/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Extracellular Fluid/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Manganese/metabolism
MH  - Membrane Proteins/*blood
MH  - Neoplasm Proteins/*blood
MH  - ORAI1 Protein
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases/antagonists & 
      inhibitors/blood
MH  - Stromal Interaction Molecule 1
MH  - Thapsigargin/pharmacology
EDAT- 2009/07/28 09:00
MHDA- 2009/11/10 06:00
CRDT- 2009/07/28 09:00
PHST- 2009/05/29 00:00 [received]
PHST- 2009/07/03 00:00 [revised]
PHST- 2009/07/13 00:00 [accepted]
PHST- 2009/07/28 09:00 [entrez]
PHST- 2009/07/28 09:00 [pubmed]
PHST- 2009/11/10 06:00 [medline]
AID - S0167-4889(09)00186-4 [pii]
AID - 10.1016/j.bbamcr.2009.07.003 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2009 Oct;1793(10):1614-22. doi: 
      10.1016/j.bbamcr.2009.07.003. Epub 2009 Jul 23.