PMID- 19632308
OWN - NLM
STAT- MEDLINE
DCOM- 20100212
LR  - 20231213
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 163
IP  - 4
DP  - 2009 Nov 10
TI  - Differential gene expression in the rat hippocampus during learning of an operant 
      conditioning task.
PG  - 1031-8
LID - 10.1016/j.neuroscience.2009.07.037 [doi]
AB  - Changes in transcription levels of brain-derived neurotrophic factor (BDNF), 
      cyclic adenosine monophosphate (cAMP) response element binding (CREB), Synapsin 
      I, Ca(2+)/calmodulin-dependent protein kinase II (CamKII), activity-regulated 
      cytoskeleton-associated protein (Arc), c-jun and c-fos have been associated to 
      several learning paradigms in different brain areas. In this study, we measured 
      mRNA expression in the hippocampus by real time (RT)-PCR mRNA levels of BDNF, 
      CREB, Synapsin I, CamKII, Arc, c-jun and c-fos, during learning and operant 
      conditioning task. Experimental groups were as follows: control (C, the animals 
      never left the bioterium), when the animals reached 50-65% of the expected 
      response (Incompletely Trained, IT), when animals reached 100% of the expected 
      response with a latency time lower than 5 s (Trained, Tr), Box Control of 
      Incompletely Trained (BCIT), animals spent the same time as the IT in the operant 
      conditioning box and Box Control of Trained (BCTr) animals spent the same time as 
      the Tr in the operant conditioning box. All rats were killed at the same time by 
      cervical dislocation 15 min after training and hippocampi were removed and 
      processed. We found increments of mRNA levels of most genes (BDNF, CREB, Synapsin 
      I, Arc, c-jun and c-fos) in IT and Tr groups compared to their box controls, but 
      increments in Tr were smaller compared with IT. These results describe a 
      differential gene expression in the rat hippocampus when the animals are learning 
      and when animals have already learned. Taking together the results presented 
      herein with the known functions of these genes, we propose a link between changes 
      in gene expression in the hippocampus and different degrees of cellular 
      activation and plasticity during learning of an operant conditioning task.
FAU - Rapanelli, M
AU  - Rapanelli M
AD  - IBYME-CONICET, Laboratorio de Biologia del Comportamiento, Vuelta de Obligado 
      2490, Ciudad de Buenos Aires, Buenos Aires, Argentina. rapanelli@dna.uba.ar
FAU - Frick, L R
AU  - Frick LR
FAU - Zanutto, B S
AU  - Zanutto BS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090724
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Creb1 protein, rat)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Synapsins)
RN  - 0 (activity regulated cytoskeletal-associated protein)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/genetics/metabolism
MH  - Conditioning, Operant/*physiology
MH  - Cyclic AMP Response Element-Binding Protein/genetics/metabolism
MH  - Cytoskeletal Proteins/genetics/metabolism
MH  - Gene Expression
MH  - Hippocampus/*physiology
MH  - Learning/*physiology
MH  - Male
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Proto-Oncogene Proteins c-fos/genetics/metabolism
MH  - Proto-Oncogene Proteins c-jun/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Synapsins/genetics/metabolism
MH  - Time Factors
EDAT- 2009/07/28 09:00
MHDA- 2010/02/13 06:00
CRDT- 2009/07/28 09:00
PHST- 2009/04/02 00:00 [received]
PHST- 2009/07/07 00:00 [revised]
PHST- 2009/07/18 00:00 [accepted]
PHST- 2009/07/28 09:00 [entrez]
PHST- 2009/07/28 09:00 [pubmed]
PHST- 2010/02/13 06:00 [medline]
AID - S0306-4522(09)01211-1 [pii]
AID - 10.1016/j.neuroscience.2009.07.037 [doi]
PST - ppublish
SO  - Neuroscience. 2009 Nov 10;163(4):1031-8. doi: 10.1016/j.neuroscience.2009.07.037. 
      Epub 2009 Jul 24.