PMID- 1963300
OWN - NLM
STAT- MEDLINE
DCOM- 19910402
LR  - 20141120
IS  - 0004-4172 (Print)
IS  - 0004-4172 (Linking)
VI  - 40
IP  - 10
DP  - 1990 Oct
TI  - Priming effect of recombinant human interleukin-2 and recombinant human 
      interferon-gamma on human neutrophil superoxide production.
PG  - 1176-9
AB  - The effect of recombinant human interleukin-2 (rhIL-2) and recombinant human 
      interferon-gamma (rhIFN-gamma) were evaluated on superoxide (O2-) production of 
      human polymorphonuclear neutrophils (PMNs). Ten minutes incubation with rhIL-2 
      showed a dose-dependent enhancement of n-formyl-methionyl-leucyl-phenylalanine 
      (FMLP)-induced O2-production of human PMNs, and the rate of enhancement reached 
      49.6% at the concentration of 3000 U/ml rhIL-2. Same pretreatment with 
      rhIFN-gamma also showed a dose-dependent enhancement of FMLP-induced 
      O2-production of human PMNs, and the maximal rate of enhancement was 47.0% at the 
      concentration of 3000 U/ml rhIFN-gamma. Any cytokines given alone did not induce 
      O2- production. These cytokines showed no enhancement of phorbol myristate 
      acetate (PMA)-induced O2- production, neither. The effects of these cytokines to 
      FMLP-induced O2- production were kept in calcium-free medium. Moreover, 
      incubation with these cytokines caused no elevation of intracellular free calcium 
      concentration [( Ca++]i) in resting PMNs. Incubation with them did not change the 
      increase of [( Ca++]i) of PMNs induced by FMLP significantly, neither. 
      Recombinant forms of cytokines are used clinically, now. These results may be 
      helpful for the use of them.
FAU - Suzuki, K
AU  - Suzuki K
AD  - Second Department of Internal Medicine, School of Medicine, University of Nagoya, 
      Japan.
FAU - Furui, H
AU  - Furui H
FAU - Kaneko, M
AU  - Kaneko M
FAU - Takagi, K
AU  - Takagi K
FAU - Satake, T
AU  - Satake T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arzneimittelforschung
JT  - Arzneimittel-Forschung
JID - 0372660
RN  - 0 (Interleukin-2)
RN  - 0 (Phorbol Esters)
RN  - 0 (Receptors, Granulocyte Colony-Stimulating Factor)
RN  - 0 (Recombinant Proteins)
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 82115-62-6 (Interferon-gamma)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Calcium/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferon-gamma/*pharmacology
MH  - Interleukin-2/*pharmacology
MH  - Kinetics
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*metabolism
MH  - Phorbol Esters
MH  - Receptors, Granulocyte Colony-Stimulating Factor
MH  - Recombinant Proteins/pharmacology
MH  - Superoxides/*metabolism
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
PST - ppublish
SO  - Arzneimittelforschung. 1990 Oct;40(10):1176-9.