PMID- 19634013
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20090806
IS  - 1573-675X (Electronic)
IS  - 1360-8185 (Linking)
VI  - 14
IP  - 9
DP  - 2009 Sep
TI  - Cell death induced by N-methyl-N-nitrosourea, a model S(N)1 methylating agent, in 
      two lung cancer cell lines of human origin.
PG  - 1121-33
LID - 10.1007/s10495-009-0379-x [doi]
AB  - New therapeutic approaches are needed for lung cancer, the leading cause of 
      cancer death. Methylating agents constitute a widely used class of anticancer 
      drugs, the effect of which on human non small cell lung cancer (NSCLC) has not 
      been adequately studied. N-methyl-N-nitrosourea (MNU), a model S(N)1 methylating 
      agent, induced cell death through a distinct mechanism in two human NSCLC cell 
      lines studied, A549(p53(wt)) and H157(p53(null)). In A549(p53(wt)), MNU induced 
      G2/M arrest, accompanied by cdc25A degradation, hnRNP B1 induction, hnRNP C1/C2 
      downregulation. Non-apoptotic cell death was confirmed by the lack of increase in 
      the sub-G1 DNA content, Poly (ADP-ribose) polymerase cleavage and caspase-3, -7 
      activation. In H157(p53(null)), MNU induced apoptotic cell death, confirmed by 
      cytofluorometry of DNA content and immunodetection of apoptotic markers, 
      accompanied by overexpression of hnRNP B1 and C1/C2. Thus, the mechanism of the 
      cell death induced by S(N)1 methylating agents is cell type-dependent and must be 
      assessed prior treatment.
FAU - Koryllou, Angeliki
AU  - Koryllou A
AD  - Institute of Biological Research and Biotechnology, National Hellenic Research 
      Foundation, Athens, Greece.
FAU - Patrinou-Georgoula, Meropi
AU  - Patrinou-Georgoula M
FAU - Troungos, Constantinos
AU  - Troungos C
FAU - Pletsa, Vassiliki
AU  - Pletsa V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Apoptosis
JT  - Apoptosis : an international journal on programmed cell death
JID - 9712129
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (Indoles)
RN  - 0 (Neoplasm Proteins)
RN  - 36015-30-2 (Propidium)
RN  - 47165-04-8 (DAPI)
RN  - 684-93-5 (Methylnitrosourea)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - Blotting, Western
MH  - Carcinoma, Non-Small-Cell Lung/*pathology
MH  - Cell Cycle/drug effects
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cell Survival/drug effects
MH  - Heterogeneous-Nuclear Ribonucleoproteins/metabolism
MH  - Humans
MH  - Indoles/metabolism
MH  - Lung Neoplasms/*pathology
MH  - Methylnitrosourea/*pharmacology
MH  - Microscopy, Confocal
MH  - Neoplasm Proteins/metabolism
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Propidium/metabolism
MH  - Staining and Labeling
MH  - Tumor Stem Cell Assay
EDAT- 2009/07/28 09:00
MHDA- 2009/10/22 06:00
CRDT- 2009/07/28 09:00
PHST- 2009/07/28 09:00 [entrez]
PHST- 2009/07/28 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
AID - 10.1007/s10495-009-0379-x [doi]
PST - ppublish
SO  - Apoptosis. 2009 Sep;14(9):1121-33. doi: 10.1007/s10495-009-0379-x.