PMID- 19634869
OWN - NLM
STAT- MEDLINE
DCOM- 20100715
LR  - 20121115
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 57
IP  - 16
DP  - 2009 Aug 26
TI  - Xanthohumol from hop (Humulus lupulus L.) is an efficient inhibitor of monocyte 
      chemoattractant protein-1 and tumor necrosis factor-alpha release in 
      LPS-stimulated RAW 264.7 mouse macrophages and U937 human monocytes.
PG  - 7274-81
LID - 10.1021/jf901244k [doi]
AB  - Activated macrophages in adipose tissue play a major role in the chronic 
      inflammatory process that has been linked to the complications of overweight and 
      obesity. The hop plant (Humulus lupulus L.) has been described to possess both 
      anti-inflammatory and antidiabetic effects. In the present study, the chemical 
      composition of a hop crude extract (HCE) was investigated by 
      ultrahigh-performance liquid chromatography (UHPLC). Next, HCE and various 
      fractions obtained by preparative HPLC were tested for their ability to inhibit 
      production of two pro-inflammatory cytokines, monocyte chemoattractant protein-1 
      (MCP-1, CCL2) and tumor necrosis factor-alpha (TNF-alpha), which play crucial 
      roles in the complications of obesity. The hop chalcone xanthohumol was found to 
      be the most potent inhibitor of both cytokines in LPS-activated RAW 264.7 mouse 
      macrophages and U937 human monocytes. Moreover, other constituents, namely, 
      iso-alpha-acids, in combination with the beta-acid hulupone, showed a moderate 
      but selective inhibitory activity only on MCP-1 release. These findings 
      underscore the potential health effects of hop and support further optimization, 
      selection, and use of this plant.
FAU - Lupinacci, Elvira
AU  - Lupinacci E
AD  - Division of Human Nutrition, Wageningen University, Wageningen, The Nethelands.
FAU - Meijerink, Jocelijn
AU  - Meijerink J
FAU - Vincken, Jean-Paul
AU  - Vincken JP
FAU - Gabriele, Bartolo
AU  - Gabriele B
FAU - Gruppen, Harry
AU  - Gruppen H
FAU - Witkamp, Renger F
AU  - Witkamp RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Chemokine CCL2)
RN  - 0 (Flavonoids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Propiophenones)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - T4467YT1NT (xanthohumol)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/*immunology
MH  - *Down-Regulation
MH  - Flavonoids/*immunology
MH  - Humans
MH  - Humulus/chemistry/*immunology
MH  - Lipopolysaccharides/*immunology
MH  - Macrophages/*drug effects/immunology
MH  - Mice
MH  - Monocytes/*drug effects/immunology
MH  - Propiophenones/*immunology
MH  - Tumor Necrosis Factor-alpha/*immunology
EDAT- 2009/07/29 09:00
MHDA- 2010/07/16 06:00
CRDT- 2009/07/29 09:00
PHST- 2009/07/29 09:00 [entrez]
PHST- 2009/07/29 09:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
AID - 10.1021/jf901244k [doi]
PST - ppublish
SO  - J Agric Food Chem. 2009 Aug 26;57(16):7274-81. doi: 10.1021/jf901244k.