PMID- 19638147
OWN - NLM
STAT- MEDLINE
DCOM- 20090902
LR  - 20220408
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 58
IP  - 5
DP  - 2009 Jun
TI  - Delayed fluorescence as a universal tool for the measurement of circadian rhythms 
      in higher plants.
PG  - 893-901
LID - 10.1111/j.1365-313X.2009.03819.x [doi]
AB  - The plant circadian clock plays an important role in enhancing performance and 
      increasing vegetative yield. Much of our current understanding of the mechanism 
      and function of the plant clock has come from the development of Arabidopsis 
      thaliana as a model circadian organism. Key to this rapid progress has been the 
      development of robust circadian markers, specifically circadian-regulated 
      luciferase reporter genes. Studies of the clock in crop species and non-model 
      organisms are currently hindered by the absence of a simple high-throughput 
      universal assay for clock function, accuracy and robustness. Delayed fluorescence 
      (DF) is a fundamental process occurring in all photosynthetic organisms. It is 
      luminescence-produced post-illumination due to charge recombination in 
      photosystem II (PSII) leading to excitation of P680 and the subsequent emission 
      of a photon. Here we report that the amount of DF oscillates with an 
      approximately 24-h period and is under the control of the circadian clock in a 
      diverse selection of plants. Thus, DF provides a simple clock output that may 
      allow the clock to be assayed in vivo in any photosynthetic organism. 
      Furthermore, our data provide direct evidence that the nucleus-encoded, 
      three-loop circadian oscillator underlies rhythms of PSII activity in the 
      chloroplast. This simple, high-throughput and non-transgenic assay could be 
      integrated into crop breeding programmes, the assay allows the selection of 
      plants that have robust and accurate clocks, and possibly enhanced performance 
      and vegetative yield. This assay could also be used to characterize rapidly the 
      role and function of any novel Arabidopsis circadian mutant.
FAU - Gould, Peter D
AU  - Gould PD
AD  - School of Biological Sciences, University of Liverpool, Crown Street, Liverpool, 
      UK.
FAU - Diaz, Patrick
AU  - Diaz P
FAU - Hogben, Claire
AU  - Hogben C
FAU - Kusakina, Jelena
AU  - Kusakina J
FAU - Salem, Radia
AU  - Salem R
FAU - Hartwell, James
AU  - Hartwell J
FAU - Hall, Anthony
AU  - Hall A
LA  - eng
GR  - BB/F009313/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BBS/B/11125/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Photosystem II Protein Complex)
SB  - IM
MH  - Arabidopsis/genetics/physiology
MH  - *Biological Clocks
MH  - Chloroplasts/physiology
MH  - *Circadian Rhythm
MH  - *Fluorescence
MH  - Magnoliopsida/*physiology
MH  - Photoperiod
MH  - Photosystem II Protein Complex/physiology
MH  - Promoter Regions, Genetic
EDAT- 2009/07/30 09:00
MHDA- 2009/09/03 06:00
CRDT- 2009/07/30 09:00
PHST- 2009/07/30 09:00 [entrez]
PHST- 2009/07/30 09:00 [pubmed]
PHST- 2009/09/03 06:00 [medline]
AID - TPJ3819 [pii]
AID - 10.1111/j.1365-313X.2009.03819.x [doi]
PST - ppublish
SO  - Plant J. 2009 Jun;58(5):893-901. doi: 10.1111/j.1365-313X.2009.03819.x.