PMID- 19639225
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20190606
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 24
IP  - 3
DP  - 2009 Sep
TI  - Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells 
      detected by three dimensional molecular cytogenetics.
PG  - 335-41
AB  - The impact of chromosome architecture in the formation of chromosome aberrations 
      is a recent finding of interphase directed molecular cytogenetic studies. Also 
      positive correlation of translocation frequencies and spatial proximity of 
      chromosomes was described. Thus, disease specific chromosomal translocations 
      could be due to tissue specific genomic organization. However, no 
      three-dimensional interphase fluorescence in situ hybridization (FISH) studies 
      for the nuclear architecture of bone marrow (BM) cells have previously been done. 
      In this study, BM of three secondary acute myelogenous leukemia (AML) cases with 
      trisomy 8 and otherwise normal karyotype were evaluated. Bone marrow cells of one 
      AML and one ALL (acute lymphoblastic leukemia) case, peripheral blood lymphocytes 
      and human sperm, all of them with normal karyotype, served as controls. 
      Multicolor banding (MCB) probes for chromosomes 8 and 21 were applied in 
      suspension-FISH (S-FISH). Interestingly, in myeloid bone marrow cells chromosomes 
      8 (di- and trisomic) and 21 tended to co-localize with their homologue 
      chromosome(s), rather than to be separated. Thus, the co-localization of 
      chromosomes 8 and 21 might promote a translocation providing a selective 
      advantage of t(8;21) cells in AML-M2. In summary, the concept that tissue 
      specific spatial proximity of chromosomes leads to enhanced translocation 
      frequencies was further supported.
FAU - Manvelyan, Marina
AU  - Manvelyan M
AD  - Jena University Hospital, Institute of Human Genetics and Anthropology, Jena, 
      Germany.
FAU - Kempf, Philipp
AU  - Kempf P
FAU - Weise, Anja
AU  - Weise A
FAU - Mrasek, Kristin
AU  - Mrasek K
FAU - Heller, Anita
AU  - Heller A
FAU - Lier, Amelie
AU  - Lier A
FAU - Hoffken, Klaus
AU  - Hoffken K
FAU - Fricke, Hans-Jorg
AU  - Fricke HJ
FAU - Sayer, Herbert G
AU  - Sayer HG
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Mkrtchyan, Hasmik
AU  - Mkrtchyan H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
SB  - IM
MH  - Adult
MH  - Aged, 80 and over
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Chromosomes, Human, Pair 21/*metabolism
MH  - Chromosomes, Human, Pair 8/*metabolism
MH  - Cytogenetic Analysis/*methods
MH  - Humans
MH  - In Situ Hybridization
MH  - Interphase
MH  - Male
MH  - Middle Aged
MH  - Myeloid Cells/cytology/*metabolism
EDAT- 2009/07/30 09:00
MHDA- 2009/10/01 06:00
CRDT- 2009/07/30 09:00
PHST- 2009/07/30 09:00 [entrez]
PHST- 2009/07/30 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
AID - 10.3892/ijmm_00000237 [doi]
PST - ppublish
SO  - Int J Mol Med. 2009 Sep;24(3):335-41. doi: 10.3892/ijmm_00000237.