PMID- 19645241
OWN - NLM
STAT- MEDLINE
DCOM- 20091229
LR  - 20090803
IS  - 1001-4454 (Print)
IS  - 1001-4454 (Linking)
VI  - 32
IP  - 4
DP  - 2009 Apr
TI  - [The effect of the type II collagen protein from Zaocys on cytokines production 
      by synoviocytes in rats with adjuvant arthritis].
PG  - 556-60
AB  - OBJECTIVE: To investigate the effect of the type II collagen (C II) protein from 
      Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis 
      (AA). METHODS: Type II collagen protein was abstracted and purificated from 
      Zaocys. Adjuvant arthritis (AA) was induced by a single intradermal injection of 
      0.1 mL of complete Freund's adjuvant into the left hind paw. Synoviocytes' 
      supernatants were harvested and synoviocytes-Peyer's Patches (PP) cells coculture 
      system were developed. Tumor necrosis factor-alpha (TNF-alpha) activity was 
      measured by L929 cytotoxicity bioassay and Interleukin (IL)-1beta activity was 
      measured by MTT dye reduction. The synoviocytes' supernatants cytokines' levels 
      were detected by ELISA. RESULTS: Each concentration of C II from Zaocys had no 
      effect on IL-1beta and TNF-alpha production by synoviocytes in vitro. Middle 
      concentration of C II suppressed the activity of IL-1beta and TNF-alpha 
      production by synoviocytes-PP cells coculture system (P < 0.05). Treating with 
      low and high dose of C II suppressed the activity of TNF-alpha and IL-1beta 
      producing by synoviocyte (P < 0.05), significantly suppressed in the group of AA 
      rats treated with middle dose of C II (P < 0.01). Treating with middle and high 
      dose of C II decreased the level of synoviocytes' supernatants TNF-alpha (P < 
      0.05), the level of synoviocytes' supernatants IL-1beta decreased in all treating 
      groups (P < 0.05). Treating with middle dose of C II increased the level of serum 
      TGF-beta (P < 0.05). Middle concentration of C II suppressed the activity of IL-1 
      and TNF production by synoviocytes-PP cells coculture system (P < 0.05). 
      CONCLUSIONS: C II from Zaocys has no direct effect on the activity of IL-1beta 
      and TNF production by synoviocytes in vitro. Oral administration of type II 
      collagen protein from Zaocys can effectively suppressed the activity and level of 
      the cytokines production by synoviocytes in rats with adjuvant arthritis (AA).
FAU - Pang, Jie
AU  - Pang J
AD  - Department of Rheumatic, Southern Medical University, Guangzhou 510515, China. 
      pangjie27@hotmail.com
FAU - Li, Juan
AU  - Li J
FAU - Wu, Xiang-Hui
AU  - Wu XH
FAU - Wu, He-Yong
AU  - Wu HY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Yao Cai
JT  - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
JID - 9426370
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Collagen Type II)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Materia Medica)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/administration & dosage/pharmacology/therapeutic use
MH  - Arthritis, Experimental/chemically induced/*drug therapy/metabolism
MH  - Cells, Cultured
MH  - Collagen Type II/administration & dosage/*pharmacology/therapeutic use
MH  - *Colubridae
MH  - Cytokines/drug effects/*metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Interleukin-1/metabolism
MH  - Materia Medica/administration & dosage/*pharmacology/therapeutic use
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synovial Membrane/*drug effects/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/drug effects/metabolism
EDAT- 2009/08/04 09:00
MHDA- 2009/12/30 06:00
CRDT- 2009/08/04 09:00
PHST- 2009/08/04 09:00 [entrez]
PHST- 2009/08/04 09:00 [pubmed]
PHST- 2009/12/30 06:00 [medline]
PST - ppublish
SO  - Zhong Yao Cai. 2009 Apr;32(4):556-60.