PMID- 19645472
OWN - NLM
STAT- MEDLINE
DCOM- 20091229
LR  - 20131121
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Linking)
VI  - 131
IP  - 33
DP  - 2009 Aug 26
TI  - Identification of a radical intermediate in the enzymatic reduction of oxygen by 
      a small laccase.
PG  - 11680-2
LID - 10.1021/ja900751c [doi]
AB  - The enzyme mechanism of the Cu-containing small laccase (SLAC) from Streptomyces 
      coelicolor has been investigated by optical and electron paramagnetic resonance 
      spectroscopy. A new intermediate was identified after the reaction of molecular 
      oxygen with the reduced trinuclear site of the type-1-depleted (T1D) form of the 
      enzyme. It has the fingerprint of a biradical with a triplet ground state. One of 
      the spins resides on a Cu in the trinuclear site, tentatively identified as the 
      type-2 site, while the other spin derives from a protein-based radical. The 
      latter is tentatively identified as a tyrosyl radical on the basis of the 
      similarity of the optical characteristics with those observed for a Cu tyrosyl 
      radical pair. The spin-spin distance was found to be 5.0 +/- 0.2 A.
FAU - Tepper, Armand W J W
AU  - Tepper AW
AD  - Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
FAU - Milikisyants, Sergey
AU  - Milikisyants S
FAU - Sottini, Silvia
AU  - Sottini S
FAU - Vijgenboom, Erik
AU  - Vijgenboom E
FAU - Groenen, Edgar J J
AU  - Groenen EJ
FAU - Canters, Gerard W
AU  - Canters GW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Free Radicals)
RN  - EC 1.10.3.2 (Laccase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Absorption
MH  - Catalytic Domain
MH  - Electron Spin Resonance Spectroscopy
MH  - Free Radicals/analysis/metabolism
MH  - Laccase/chemistry/*metabolism
MH  - Models, Molecular
MH  - Oxidation-Reduction
MH  - Oxygen/*metabolism
MH  - Sordariales/enzymology
MH  - Spectrum Analysis
EDAT- 2009/08/04 09:00
MHDA- 2009/12/30 06:00
CRDT- 2009/08/04 09:00
PHST- 2009/08/04 09:00 [entrez]
PHST- 2009/08/04 09:00 [pubmed]
PHST- 2009/12/30 06:00 [medline]
AID - 10.1021/ja900751c [doi]
PST - ppublish
SO  - J Am Chem Soc. 2009 Aug 26;131(33):11680-2. doi: 10.1021/ja900751c.