PMID- 19645846
OWN - NLM
STAT- MEDLINE
DCOM- 20091109
LR  - 20161018
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 23
IP  - 3
DP  - 2009 May-Jun
TI  - Reproducibility of airway responsiveness in horses using flowmetric 
      plethysmography and histamine bronchoprovocation.
PG  - 631-5
LID - 10.1111/j.1939-1676.2009.0307.x [doi]
AB  - BACKGROUND: Inflammatory airway disease has a high prevalence in horses, but is 
      often a diagnostic challenge. Flowmetric plethysmography and histamine 
      bronchoprovocation (FP/HBP) is a simple and effective tool for diagnosis, but 
      reproducibility of these measurements made over time has not been established. 
      HYPOTHESIS: We hypothesize that the measurement of airway responsiveness in 
      horses using FP/HBP is consistent over both short and long periods of time. 
      ANIMALS: Twenty-nine healthy adult horses from 2 university herds. METHODS: In 
      this prospective experimental study, airway responsiveness was determined in each 
      horse at day 0 (baseline [BL]) with FP/ HBP, using PC35 (provocative 
      concentration of histamine needed to increase Delta(flow) by 35%) as a measure of 
      airway responsiveness. Each horse was re-tested 1-4 weeks after BL (short-term 
      [ST]) and again at 3-12 months after BL (long-term [LT]). RESULTS: In the ST 
      period, 23/27 (85%) of the horses had a PC35 that was within 1 doubling 
      concentration of histamine of their BL value, with a mean change of 0.52 doubling 
      concentrations (95% CI 0.26-0.79, range 0-2.06). For the LT data, 19/26 (73%) of 
      horses were within 1 doubling concentration of their BL value, with a mean change 
      of 0.81 doubling concentrations (95% CI 0.45-1.17, range 0.14-3.10). There was no 
      significant difference in reproducibility between the 2 groups of subjects. 
      CONCLUSIONS AND CLINICAL IMPORTANCE: Repeated measurements of airway 
      responsiveness obtained with FP/HBP show acceptable reproducibility over time 
      periods up to a year. However, caution must be used when testing horses when 
      ambient air temperature is low.
FAU - Nolen-Walston, R D
AU  - Nolen-Walston RD
AD  - Department of Clinical Studies, New Bolton Center, University of Pennsylvania, 
      Kennett Square, PA 19348, USA. rnolenw@vet.upenn.edu
FAU - Kuehn, H
AU  - Kuehn H
FAU - Boston, R C
AU  - Boston RC
FAU - Mazan, M R
AU  - Mazan MR
FAU - Wilkins, P A
AU  - Wilkins PA
FAU - Bruns, S
AU  - Bruns S
FAU - Hoffman, A M
AU  - Hoffman AM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Histamine/*toxicity
MH  - Horse Diseases/*chemically induced
MH  - Horses
MH  - Plethysmography/methods/*veterinary
MH  - Reproducibility of Results
MH  - Respiratory Hypersensitivity/diagnosis/*veterinary
EDAT- 2009/08/04 09:00
MHDA- 2009/11/10 06:00
CRDT- 2009/08/04 09:00
PHST- 2009/08/04 09:00 [entrez]
PHST- 2009/08/04 09:00 [pubmed]
PHST- 2009/11/10 06:00 [medline]
AID - JVIM307 [pii]
AID - 10.1111/j.1939-1676.2009.0307.x [doi]
PST - ppublish
SO  - J Vet Intern Med. 2009 May-Jun;23(3):631-5. doi: 10.1111/j.1939-1676.2009.0307.x.