PMID- 19645915
OWN - NLM
STAT- MEDLINE
DCOM- 20090818
LR  - 20090803
IS  - 1749-6632 (Electronic)
IS  - 0077-8923 (Linking)
VI  - 1164
DP  - 2009 May
TI  - The presence of lytic HSV-1 transcripts and clonally expanded T cells with a 
      memory effector phenotype in human sensory ganglia.
PG  - 300-4
LID - 10.1111/j.1749-6632.2009.03871.x [doi]
AB  - Herpes simplex virus type 1 (HSV-1) latent persistence in human trigeminal 
      ganglia (TG) is accompanied by a chronic CD8 T-cell infiltration. Thus far, 
      during HSV-1 latency only a single transcript, namely the latency-associated 
      transcript (LAT), has been identified to be synthesized but not translated into a 
      protein. In contrast, the chronic CD8 T-cell infiltration suggests that an 
      antigen trigger must be present. The focus of the current work was to look for 
      HSV-1 transcription activity as a potential trigger of the immune response and to 
      demonstrate whether the immune cells are clonally expanded and have a phenotype 
      that suggests that they have been triggered by viral antigen. By combining in 
      situ hybridization, laser cutting microscopy, and single-cell real time RT-PCR, 
      we demonstrated expression of the HSV-1 immediate early (IE) genes ICP0 and ICP4 
      in human trigeminal neurons. Using CDR3 spectratyping, we showed that the 
      infiltrating T cells are clonally expanded, indicating an antigen-driven immune 
      response. Moreover, the persisting CD8(+) T cells had prominent features of the 
      memory effector phenotype. Chemokines CCL5 and CXCL10 were expressed by a 
      subpopulation of infiltrating cells and the corresponding chemokine receptors 
      CCR5 and CXCR3 were co-expressed on virtually all T cells bearing the CD8 
      phenotype. Thus, HSV-1 IE genes are expressed in human TG, and the infiltrating T 
      cells bear several characteristics that suggest viral antigenic stimulation.
FAU - Derfuss, Tobias
AU  - Derfuss T
AD  - Department of Neurology, Klinikum Grosshadern, Ludwig-Maximilians University, 
      Munich, Germany.
FAU - Arbusow, Viktor
AU  - Arbusow V
FAU - Strupp, Michael
AU  - Strupp M
FAU - Brandt, Thomas
AU  - Brandt T
FAU - Theil, Diethilde
AU  - Theil D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Ganglia, Sensory/*immunology/*virology
MH  - Genes, Immediate-Early
MH  - Genes, Viral
MH  - Herpesvirus 1, Human/*genetics
MH  - Humans
MH  - *Immunologic Memory
MH  - In Situ Hybridization
MH  - RNA, Messenger/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/*immunology
EDAT- 2009/08/04 09:00
MHDA- 2009/08/19 09:00
CRDT- 2009/08/04 09:00
PHST- 2009/08/04 09:00 [entrez]
PHST- 2009/08/04 09:00 [pubmed]
PHST- 2009/08/19 09:00 [medline]
AID - NYAS03871 [pii]
AID - 10.1111/j.1749-6632.2009.03871.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2009 May;1164:300-4. doi: 10.1111/j.1749-6632.2009.03871.x.