PMID- 19646827
OWN - NLM
STAT- MEDLINE
DCOM- 20100324
LR  - 20211020
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 140
IP  - 1-2
DP  - 2010 Jan 6
TI  - Development and use of real-time PCR to detect and quantify Mycoplasma haemocanis 
      and "Candidatus Mycoplasma haematoparvum" in dogs.
PG  - 167-70
LID - 10.1016/j.vetmic.2009.07.006 [doi]
AB  - Two canine haemoplasma species have been recognised to date; Mycoplasma 
      haemocanis (Mhc), which has been associated with anaemia in splenectomised or 
      immunocompromised dogs, and "Candidatus Mycoplasma haematoparvum" (CMhp), 
      recently described in an anaemic splenectomised dog undergoing chemotherapy. The 
      study aim was to develop quantitative real-time PCR assays (qPCRs) incorporating 
      an endogenous internal control to detect Mhc and CMhp and to apply these assays 
      to DNA samples extracted from canine blood collected in Northern Tanzania (n=100) 
      and from dogs presented to a Trinidadian veterinary hospital (n=185). QPCRs 
      specific for Mhc and CMhp were designed using 16S rRNA gene sequence data, and 
      each was duplexed with an assay specific for canine glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH). The assays detected < or =10 copies of a sequence-specific 
      haemoplasma plasmid per reaction and neither assay showed cross-reactivity with 
      10(6) copies of the sequence-specific plasmid from the non-target canine 
      haemoplasma species. Nineteen of the 100 Tanzanian samples (19%) were positive 
      for Mhc alone and one (1%) was dually infected. One Trinidadian sample was 
      negative for canine GAPDH DNA and was excluded from the study. Of the 184 
      remaining Trinidadian samples, nine (4.9%) were positive for Mhc alone, five 
      (2.7%) for CMhp alone, and two (1.1%) dually infected. This is the first report 
      of canine haemoplasma qPCR assays that use an internal control to confirm the 
      presence of amplifiable sample DNA, and their application to prevalence studies. 
      Mhc was the most commonly detected canine haemoplasma species.
FAU - Barker, E N
AU  - Barker EN
AD  - Department of Clinical Veterinary Science, University of Bristol, Langford House, 
      Langford, Bristol, BS40 5DU, UK. emi.barker@bristol.ac.uk
FAU - Tasker, S
AU  - Tasker S
FAU - Day, M J
AU  - Day MJ
FAU - Warman, S M
AU  - Warman SM
FAU - Woolley, K
AU  - Woolley K
FAU - Birtles, R
AU  - Birtles R
FAU - Georges, K C
AU  - Georges KC
FAU - Ezeokoli, C D
AU  - Ezeokoli CD
FAU - Newaj-Fyzul, A
AU  - Newaj-Fyzul A
FAU - Campbell, M D
AU  - Campbell MD
FAU - Sparagano, O A E
AU  - Sparagano OA
FAU - Cleaveland, S
AU  - Cleaveland S
FAU - Helps, C R
AU  - Helps CR
LA  - eng
GR  - 077718/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
DEP - 20090710
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - DNA, Bacterial/analysis/blood/genetics
MH  - Dog Diseases/*diagnosis/*epidemiology/microbiology
MH  - Dogs
MH  - Female
MH  - Male
MH  - Mycoplasma/genetics/isolation & purification/*physiology
MH  - Mycoplasma Infections/diagnosis/epidemiology/*veterinary
MH  - Polymerase Chain Reaction/methods/*veterinary
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sensitivity and Specificity
MH  - Tanzania/epidemiology
MH  - Trinidad and Tobago/epidemiology
PMC - PMC2805721
EDAT- 2009/08/04 09:00
MHDA- 2010/03/25 06:00
PMCR- 2010/01/06
CRDT- 2009/08/04 09:00
PHST- 2009/03/25 00:00 [received]
PHST- 2009/07/01 00:00 [revised]
PHST- 2009/07/03 00:00 [accepted]
PHST- 2009/08/04 09:00 [entrez]
PHST- 2009/08/04 09:00 [pubmed]
PHST- 2010/03/25 06:00 [medline]
PHST- 2010/01/06 00:00 [pmc-release]
AID - S0378-1135(09)00336-8 [pii]
AID - 10.1016/j.vetmic.2009.07.006 [doi]
PST - ppublish
SO  - Vet Microbiol. 2010 Jan 6;140(1-2):167-70. doi: 10.1016/j.vetmic.2009.07.006. 
      Epub 2009 Jul 10.