PMID- 19650809
OWN - NLM
STAT- MEDLINE
DCOM- 20091117
LR  - 20090909
IS  - 1399-6576 (Electronic)
IS  - 0001-5172 (Linking)
VI  - 53
IP  - 9
DP  - 2009 Oct
TI  - Human neural stem cell transplantation attenuates apoptosis and improves 
      neurological functions after cerebral ischemia in rats.
PG  - 1184-91
LID - 10.1111/j.1399-6576.2009.02024.x [doi]
AB  - BACKGROUND: Neuroprotection is a major therapeutic approach for ischemic brain 
      injury. We investigated the neuroprotective effects induced by transplantation of 
      human embryonic neural stem cells (NSCs) into the cortical penumbra 24 h after 
      focal cerebral ischemia. METHODS: NSCs were prepared from human embryonic brains 
      obtained at 8 weeks of gestation. Focal cerebral ischemia was induced in adult 
      rats by permanent occlusion of the middle cerebral artery. Animals were randomly 
      divided into two groups: NSCs-grafted group and medium-grafted group (control). 
      Infarct size was assessed 28 days after transplantation by hematoxylin and eosin 
      staining. Neurological severity scores were evaluated before ischemia and at 1, 
      7, 14, and 28 days after transplantation. The terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay and immunohistochemical 
      analysis of Bcl-2 and Bax were performed at 7, 14, and 28 days after 
      transplantation. RESULTS: Physiological parameters of the two groups were 
      comparable, but not significantly different. NSC transplantation significantly 
      improved neurological function (P<0.05) but did not reduce the infarct size 
      significantly (P>0.05). Compared with the control, NSC transplantation 
      significantly reduced the number of TUNEL- and Bax-positive cells in the penumbra 
      at 7 days. Interestingly, the number of Bcl-2-positive cells in the penumbra 
      after NSC transplantation was significantly higher than that after medium 
      transplantation (P<0.05). CONCLUSIONS: The results indicate that NSC 
      transplantation has anti-apoptotic activity and can improve the neurological 
      function; these effects are mediated by the up-regulation of Bcl-2 expression in 
      the penumbra.
FAU - Zhang, P
AU  - Zhang P
AD  - Institute of Neurobiology, National Key Academic Subject of Physiology, Xi'an 
      Jiaotong University School of Medicine, Xi'an, China.
FAU - Li, J
AU  - Li J
FAU - Liu, Y
AU  - Liu Y
FAU - Chen, X
AU  - Chen X
FAU - Kang, Q
AU  - Kang Q
FAU - Zhao, J
AU  - Zhao J
FAU - Li, W
AU  - Li W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090722
PL  - England
TA  - Acta Anaesthesiol Scand
JT  - Acta anaesthesiologica Scandinavica
JID - 0370270
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Behavior, Animal/physiology
MH  - Brain Ischemia/pathology/*physiopathology/*therapy
MH  - Cerebral Cortex/blood supply/pathology/physiopathology
MH  - Cerebral Infarction/pathology
MH  - DNA Fragmentation
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Nervous System/*physiopathology
MH  - Proto-Oncogene Proteins c-bcl-2/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Stem Cell Transplantation
MH  - bcl-2-Associated X Protein/biosynthesis/genetics
EDAT- 2009/08/05 09:00
MHDA- 2009/11/18 06:00
CRDT- 2009/08/05 09:00
PHST- 2009/08/05 09:00 [entrez]
PHST- 2009/08/05 09:00 [pubmed]
PHST- 2009/11/18 06:00 [medline]
AID - AAS2024 [pii]
AID - 10.1111/j.1399-6576.2009.02024.x [doi]
PST - ppublish
SO  - Acta Anaesthesiol Scand. 2009 Oct;53(9):1184-91. doi: 
      10.1111/j.1399-6576.2009.02024.x. Epub 2009 Jul 22.