PMID- 1965109
OWN - NLM
STAT- MEDLINE
DCOM- 19910523
LR  - 20190918
IS  - 0166-3542 (Print)
IS  - 0166-3542 (Linking)
VI  - 14
IP  - 4-5
DP  - 1990 Oct-Nov
TI  - Evaluation of the toxicity and antiviral activity of carbocyclic 3-deazaadenosine 
      against respiratory syncytial and parainfluenza type 3 viruses in tissue culture 
      and in cotton rats.
PG  - 215-25
AB  - The toxicity and antiviral efficacy of carbocyclic 3-deazaadenosine (Cc3Ado) 
      against respiratory syncytial (RSV) and parainfluenza type 3 (PIV3) virus 
      infections were tested in tissue culture and in cotton rats. The mean median 
      efficacious dose (ED50) of Cc3Ado in HEp2 cells against RSV and PIV3 was 9 and 14 
      micrograms/ml, respectively. These values were 85- and 55-fold less than the 
      median inhibitory (toxic) dose (ID50) of Cc3Ado in this cell line (750 
      micrograms/ml), and similar to values obtained for ribavirin. Cc3Ado exhibited no 
      significant antiviral activity against influenza A, influenza B, adeno type 5 or 
      adeno type 7 viruses (all ED50 were greater than 1000 micrograms/ml). In cotton 
      rats, animals given greater than or equal to 1 mg/kg/day Cc3Ado intraperitoneally 
      on days 1, 2 and 3 after experimental challenge with virus, consistently had 
      significant reductions in pulmonary RSV and PIV3 titers compared to pulmonary 
      virus titers in comparably treated control animals. The minimum efficacious dose 
      of ribavirin given under the same conditions was 30 mg/kg/day. Cc3Ado was also 
      efficacious in cotton rats when given orally by gavage, or when different 
      administration schedules were used. The median efficacious dose of Cc3Ado when 
      given orally was 10 mg/kg/day. No significant toxic effects were noted in cotton 
      rats, even in animals given 20 mg/kg daily for eight consecutive days.
FAU - Wyde, P R
AU  - Wyde PR
AD  - Department of Microbiology and Immunology, Baylor College of Medicine, Houston, 
      Texas 77030.
FAU - Ambrose, M W
AU  - Ambrose MW
FAU - Meyer, H L
AU  - Meyer HL
FAU - Zolinski, C L
AU  - Zolinski CL
FAU - Gilbert, B E
AU  - Gilbert BE
LA  - eng
GR  - N01-AI-62522/AI/NIAID NIH HHS/United States
GR  - N01-AI-82519/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Antiviral Res
JT  - Antiviral research
JID - 8109699
RN  - 0 (Antiviral Agents)
RN  - 81601-33-4 (carbocyclic 3-deazaadenosine)
RN  - M351LCX45Y (Tubercidin)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*toxicity
MH  - Cell Line
MH  - Drug Evaluation, Preclinical
MH  - Female
MH  - Humans
MH  - Male
MH  - Parainfluenza Virus 3, Human/*drug effects/growth & development
MH  - Paramyxoviridae Infections/drug therapy/pathology
MH  - Rats
MH  - Respiratory Syncytial Viruses/*drug effects/growth & development
MH  - Respirovirus Infections/drug therapy/pathology
MH  - Tubercidin/*analogs & derivatives/pharmacology/toxicity
MH  - Virus Replication/*drug effects
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 0166-3542(90)90003-P [pii]
AID - 10.1016/0166-3542(90)90003-p [doi]
PST - ppublish
SO  - Antiviral Res. 1990 Oct-Nov;14(4-5):215-25. doi: 10.1016/0166-3542(90)90003-p.