PMID- 19651178
OWN - NLM
STAT- MEDLINE
DCOM- 20100129
LR  - 20151119
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 70
IP  - 12
DP  - 2009 Dec
TI  - Soluble total human leukocyte antigen class I and human leukocyte antigen-G 
      molecules in kidney and kidney/pancreas transplantation.
PG  - 995-9
LID - 10.1016/j.humimm.2009.07.016 [doi]
AB  - The expression of human leukocyte antigen (HLA)-G, a nonclassical HLA class I 
      molecule, and its soluble forms (sHLA-G) are found to improve graft acceptance. 
      In this study we investigated whether sHLA-G is the most biologically relevant 
      molecule among all types of soluble HLA class I molecules for graft acceptance. 
      We addressed this question in kidney-transplanted (n = 32) and 
      kidney/pancreas-transplanted patients (n = 29). To this end we analyzed the 
      levels of total soluble HLA class I (sHLA-I) in comparison to sHLA-G in 488 
      plasma samples procured before and serial after transplantation by specific 
      enzyme-linked immunoabsorbent assay. Samples from 126 healthy individuals served 
      as controls. Pretransplantation sHLA-I levels were significantly increased in 
      patients (p < 0.001), whereas sHLA-G levels were in the range of those of healthy 
      controls. Importantly, pretransplantation sHLA-I and sHLA-G levels did not differ 
      between the two groups. Patients with biopsy-proven rejection (n = 15) revealed 
      significantly lower sHLA-G levels before transplantation (mean +/- standard error 
      of the mean, 12.9 +/- 1.8 vs. 20.1 +/- 1.9, p = 0.013) and after transplantation 
      (p = 0.006, two-way analysis of variance) than patients without rejection (n = 
      46). In contrast, sHLA-I was slightly increased after but not before 
      transplantation in patients with rejection (p < 0.05, two-way analysis of 
      variance). Nonparametric determination analysis showed that pretransplantation 
      levels of sHLA-G < 11.5 ng/ml (sensitivity, 60%; specificity, 80.4%) were related 
      to rejection. Regarding antibody status, retransplantation, number of HLA 
      mismatches, recipient age, and recipient body mass index, multivariate analysis 
      showed that sHLA-G but not sHLA-I is an independent risk factor for graft 
      rejection. Thus high levels of sHLA-G but not of sHLA-I seem to contribute to 
      better graft acceptance after kidney or kidney/pancreas transplantation.
FAU - Rebmann, Vera
AU  - Rebmann V
AD  - Institut fur Transfusionsmedizin, Universitatsklinikum Essen, Essen, Germany. 
      vera.rebmann@uk-essen.de
FAU - Bartsch, Diana
AU  - Bartsch D
FAU - Wunsch, Andreas
AU  - Wunsch A
FAU - Mollenbeck, Petra
AU  - Mollenbeck P
FAU - Golda, Thomas
AU  - Golda T
FAU - Viebahn, Richard
AU  - Viebahn R
FAU - Grosse-Wilde, Hans
AU  - Grosse-Wilde H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20090806
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Biomarkers)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Biomarkers/blood
MH  - Female
MH  - Graft Rejection/*diagnosis/immunology
MH  - Graft Survival/*immunology
MH  - HLA Antigens/*blood
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*blood
MH  - Humans
MH  - Kidney Transplantation/*immunology
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - Multivariate Analysis
MH  - Pancreas Transplantation/*immunology
EDAT- 2009/08/05 09:00
MHDA- 2010/01/30 06:00
CRDT- 2009/08/05 09:00
PHST- 2009/07/15 00:00 [received]
PHST- 2009/07/24 00:00 [revised]
PHST- 2009/07/28 00:00 [accepted]
PHST- 2009/08/05 09:00 [entrez]
PHST- 2009/08/05 09:00 [pubmed]
PHST- 2010/01/30 06:00 [medline]
AID - S0198-8859(09)00191-8 [pii]
AID - 10.1016/j.humimm.2009.07.016 [doi]
PST - ppublish
SO  - Hum Immunol. 2009 Dec;70(12):995-9. doi: 10.1016/j.humimm.2009.07.016. Epub 2009 
      Aug 6.