PMID- 19651491
OWN - NLM
STAT- MEDLINE
DCOM- 20091102
LR  - 20151119
IS  - 1873-569X (Electronic)
IS  - 0923-1811 (Linking)
VI  - 56
IP  - 1
DP  - 2009 Oct
TI  - DNA demethylation of the perforin promoter in CD4(+) T cells from patients with 
      subacute cutaneous lupus erythematosus.
PG  - 33-6
LID - 10.1016/j.jdermsci.2009.06.010 [doi]
AB  - BACKGROUND: Recent evidence indicates that human lupus is an epigenetic disease 
      characterized by impaired T cell DNA methylation. Perforin, a cytotoxic effector 
      molecule, is overexpressed due to hypomethylation of its promoter regulatory 
      elements in CD4(+) T cells from patients with systemic erythematosus lupus (SLE). 
      However, it is unknown whether aberrant expression and methylation of perforin 
      occur in CD4(+) T cells from patients with subacute cutaneous lupus erythematosus 
      (SCLE). OBJECTIVE: We aimed to compare the perforin expression level and the 
      methylation status of the perforin promoter region in CD4(+) T cells from SCLE 
      patients and healthy controls. METHODS: We used real-time RT-PCR to compare the 
      perforin mRNA levels, and Western-blot to compare perforin protein levels in 
      CD4(+) and CD8(+) T cells from SCLE patients and healthy controls. Bisulfite 
      sequencing was used to determine the methylation status of the perforin promoter 
      region. RESULTS: Perforin is overexpressed in SCLE CD4(+) T cells. Demethylation 
      of the perforin promoter region was seen in CD4(+) T cells from patients with 
      SCLE. CONCLUSIONS: DNA demethylation at the perforin locus contributes to 
      perforin overexpression in SCLE CD4(+) T cells.
FAU - Luo, Yongqi
AU  - Luo Y
AD  - Department of Dermatology, Epigenetic Research Center, Second Xiangya Hospital, 
      Central South University, Changsha, PR China.
FAU - Zhang, Xiujuan
AU  - Zhang X
FAU - Zhao, Ming
AU  - Zhao M
FAU - Lu, Qianjin
AU  - Lu Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090803
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (PRF1 protein, human)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 0 (RNA, Messenger)
RN  - 126465-35-8 (Perforin)
SB  - IM
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/*metabolism
MH  - CD8-Positive T-Lymphocytes/*metabolism
MH  - *DNA Methylation
MH  - Female
MH  - Humans
MH  - Lupus Erythematosus, Cutaneous/*genetics
MH  - Male
MH  - Middle Aged
MH  - Perforin
MH  - Pore Forming Cytotoxic Proteins/*genetics
MH  - Promoter Regions, Genetic/genetics/physiology
MH  - RNA, Messenger/metabolism
EDAT- 2009/08/05 09:00
MHDA- 2009/11/03 06:00
CRDT- 2009/08/05 09:00
PHST- 2009/04/19 00:00 [received]
PHST- 2009/06/23 00:00 [revised]
PHST- 2009/06/28 00:00 [accepted]
PHST- 2009/08/05 09:00 [entrez]
PHST- 2009/08/05 09:00 [pubmed]
PHST- 2009/11/03 06:00 [medline]
AID - S0923-1811(09)00195-9 [pii]
AID - 10.1016/j.jdermsci.2009.06.010 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2009 Oct;56(1):33-6. doi: 10.1016/j.jdermsci.2009.06.010. Epub 
      2009 Aug 3.