PMID- 19652471
OWN - NLM
STAT- MEDLINE
DCOM- 20090922
LR  - 20190606
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 71
IP  - 7
DP  - 2009 Jul
TI  - Characterization of monocyte-derived dendritic cells from cats infected with 
      feline immunodeficiency virus.
PG  - 865-71
AB  - Dendritic cells (DCs) are professional antigen presenting cells (APCs) that 
      possess an extraordinary capacity to stimulate naive T cells and initiate a 
      primary immune response. To develop a DC-based immunotherapy for feline 
      immunodeficiency virus (FIV) infection, we carried out a study to characterize 
      DCs from FIV-infected cats and compared the observations with those obtained from 
      healthy controls. DCs were derived from adherent peripheral blood mononuclear 
      cells that had been cultivated with recombinant feline interleukin 4, granulocyte 
      macrophage colony-stimulating factor and heat-inactivated autologous plasma. 
      Various parameters, such as cell morphology, surface phenotype, endocytosis and 
      mixed leukocyte reaction (MLR), were analyzed to characterize feline DCs. 
      Monocyte-derived DCs from FIV-infected cats as well as those from healthy 
      controls showed a dendritic appearance and expressed an APC-like phenotype 
      (CD1c(+), CD80(+) and MHC class II(+)). However, the expression level of CD1a was 
      variable in the DCs derived from FIV-infected cats, although this was not the 
      case in the DCs derived from the healthy controls. DCs from the FIV-infected cats 
      retained the ability to take up dextran via the mannose receptor and also showed 
      an apparent MLR, indicating that these cells could be useful in immunotherapy. In 
      this study, monocytes obtained from FIV-infected cats could differentiate into 
      functional DCs, suggesting that they might be used in a DC-based immunotherapy 
      against FIV infection.
FAU - Mizukoshi, Fuminori
AU  - Mizukoshi F
AD  - Department of Veterinary Internal Medicine, Graduate School of Agricultural and 
      Life Sciences, The University of Tokyo, Tokyo, Japan.
FAU - Baba, Kenji
AU  - Baba K
FAU - Horiuchi, Hiroshi
AU  - Horiuchi H
FAU - Goto-Koshino, Yuko
AU  - Goto-Koshino Y
FAU - Setoguchi-Mukai, Asuka
AU  - Setoguchi-Mukai A
FAU - Fujino, Yasuhito
AU  - Fujino Y
FAU - Ohno, Koichi
AU  - Ohno K
FAU - Moore, Peter F
AU  - Moore PF
FAU - Tsujimoto, Hajime
AU  - Tsujimoto H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antigens)
SB  - IM
MH  - Animals
MH  - Antigens/metabolism
MH  - Cats
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*physiology
MH  - *Immunodeficiency Virus, Feline
MH  - Lentivirus Infections/immunology/*veterinary
MH  - T-Lymphocytes/cytology/physiology
EDAT- 2009/08/05 09:00
MHDA- 2009/09/23 06:00
CRDT- 2009/08/05 09:00
PHST- 2009/08/05 09:00 [entrez]
PHST- 2009/08/05 09:00 [pubmed]
PHST- 2009/09/23 06:00 [medline]
AID - JST.JSTAGE/jvms/71.865 [pii]
AID - 10.1292/jvms.71.865 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2009 Jul;71(7):865-71. doi: 10.1292/jvms.71.865.