PMID- 19655299
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20131121
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 24
IP  - 3
DP  - 2010 Mar
TI  - Antiinflammatory effects of a red orange extract in human keratinocytes treated 
      with interferon-gamma and histamine.
PG  - 414-8
LID - 10.1002/ptr.2973 [doi]
AB  - Red oranges are an important component of the so-called Mediterranean diet and 
      they have been used by traditional medicine for their health protective 
      properties, particularly to heal sore throat and cough, suggesting an interesting 
      antiinflammatory activity. The purpose of this study was to evaluate the 
      antiinflammatory activity of a red orange (Citrus sinensis varieties: Moro, 
      Tarocco, Sanguinello) complex (ROC), characterized by high levels of 
      anthocyanins, flavanones, hydroxycinnamic acids and ascorbic acid, on the human 
      keratinocyte line NCTC 2544 exposed to interferon-gamma (IFN-gamma) and 
      histamine. The expression of immunomodulatory membrane molecules such as 
      inter-cellular adhesion molecule-1 (ICAM-1) by Western blot analysis, and the 
      release of chemokines such as monocyte chemoattractant protein-1 (MCP-1) and 
      interleukin-8 (IL-8) through ELISA kits, were determined. ICAM-1 modulates the 
      permanence and activation of T lymphocytes in the epidermis. MCP-1 is a specific 
      chemoattractant for monocytes and dendritic cells. IL-8 is important for the 
      recruitment of both neutrophils and T lymphocytes. Addition of ROC at different 
      concentrations together with IFN-gamma and histamine induced a dose-dependent 
      inhibition of ICAM-1 expression and MCP-1 and IL-8 release. ROC shows interesting 
      antiinflammatory properties in human keratinocyte cells NCTC 2544. This natural 
      complex could have a topical employment and mitigate the consequences of some 
      skin pathologies.
CI  - (c) 2009 John Wiley & Sons, Ltd.
FAU - Cardile, Venera
AU  - Cardile V
AD  - Department of Physiological Sciences, University of Catania, V. le A. Doria 6, 
      95125 Catania, Italy. cardile@unict.it
FAU - Frasca, Giuseppina
AU  - Frasca G
FAU - Rizza, Luisa
AU  - Rizza L
FAU - Rapisarda, Paolo
AU  - Rapisarda P
FAU - Bonina, Francesco
AU  - Bonina F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Plant Extracts)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 820484N8I3 (Histamine)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Anti-Inflammatory Agents/immunology/*pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Citrus sinensis/*chemistry/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Histamine/immunology/*pharmacology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interferon-gamma/immunology/*pharmacology
MH  - Interleukin-8/metabolism
MH  - Keratinocytes/*drug effects/immunology
MH  - Plant Extracts/immunology/pharmacology
EDAT- 2009/08/06 09:00
MHDA- 2010/04/14 06:00
CRDT- 2009/08/06 09:00
PHST- 2009/08/06 09:00 [entrez]
PHST- 2009/08/06 09:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - 10.1002/ptr.2973 [doi]
PST - ppublish
SO  - Phytother Res. 2010 Mar;24(3):414-8. doi: 10.1002/ptr.2973.