PMID- 19655417
OWN - NLM
STAT- MEDLINE
DCOM- 20091116
LR  - 20161125
IS  - 0192-415X (Print)
IS  - 0192-415X (Linking)
VI  - 37
IP  - 4
DP  - 2009
TI  - Antioxidant and antiradical activities of Wu Ling Shen in a cell free system.
PG  - 815-28
AB  - The present study aimed to investigate the antioxidant and antiradical activities 
      of Wu Ling Shen, a popular medicinal fungus (Xylaria nigripes) used in 
      traditional Chinese medicine preparations. Two different X. nigripes materials, 
      the cultivated X. nigripes mycelia (XN) and a commercial X. nigripes product 
      (XNP), were used to prepare the aqueous (XN-H vs. XNP-H) and ethanol (XN-E vs. 
      XNP-E) extracts for this study. Polyphenol and total polysaccharide contents of 
      these extracts were also examined. Results showed that extracts of XN possessed 
      stronger antioxidant and antiradical activities than XNP in all tested model 
      systems. However, all extracts exhibited a weak activity in metal chelation and 
      reducing power. Total antioxidant activity of XN extracts (IC50 6.20 microg/ml 
      for XN-H and 5.41 microg/ml for XN-E), but not XNP extracts (IC50 128.13 
      microg/ml for XNP-H and 96.16 microg/ml for XNP-E), was more potent than Trolox 
      (IC50 19.64 microg/ml) and vitamin C (IC50 26.39 microg/ml). XN-E (IC50 5.12 
      microg/ml) and XNP-E (IC50 8.89 microg/ml) possessed a relatively similar potency 
      as that of positive controls (IC50 6.94 microg/ml for Trolox and 4.25 microg/ml 
      for vitamin C) in the superoxide radical scavenging activity. Although the DPPH 
      radical scavenging of XN extracts was weaker than that of Trolox and vitamin C, 
      it was about eight times more potent than that of XNP extracts. In ABTS assay, 
      both XN and XNP extracts exhibited a moderate ABTS radical scavenging activity. 
      Among the different extracts, XN-E showed the highest total flavonoid (32.69 
      mg/g) and phenol (59.75 mg/g) contents, while XNP-H (7.50% w/w) had the highest 
      level in total polysaccharide content. These results conclude that XN-E possesses 
      the most potent antioxidant and antiradical activities, and that these activities 
      could be derived from its high polyphenol content, but not the level of 
      polysaccharides.
FAU - Ko, Huey-Jiun
AU  - Ko HJ
AD  - Department of Food Science, National Chiayi University, Chiayi, Taiwan.
FAU - Song, Airong
AU  - Song A
FAU - Lai, Min-Nan
AU  - Lai MN
FAU - Ng, Lean-Teik
AU  - Ng LT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Antioxidants)
RN  - 0 (Biological Factors)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Chelating Agents)
RN  - 0 (Flavonoids)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Picrates)
RN  - 0 (Polysaccharides)
RN  - 11062-77-4 (Superoxides)
RN  - 339NCG44TV (Phenol)
RN  - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
SB  - IM
MH  - Antioxidants/*chemistry/pharmacology
MH  - Ascomycota/*chemistry
MH  - Biological Factors/*chemistry/pharmacology
MH  - Biphenyl Compounds/chemistry
MH  - Chelating Agents/chemistry/pharmacology
MH  - Flavonoids/analysis
MH  - Free Radical Scavengers/*chemistry/pharmacology
MH  - Mycelium/chemistry
MH  - Oxidation-Reduction/drug effects
MH  - Phenol/analysis
MH  - Picrates/chemistry
MH  - Polysaccharides/analysis
MH  - Superoxides/chemistry
MH  - Xylariales/*chemistry
EDAT- 2009/08/06 09:00
MHDA- 2009/11/17 06:00
CRDT- 2009/08/06 09:00
PHST- 2009/08/06 09:00 [entrez]
PHST- 2009/08/06 09:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
AID - S0192415X09007260 [pii]
AID - 10.1142/S0192415X09007260 [doi]
PST - ppublish
SO  - Am J Chin Med. 2009;37(4):815-28. doi: 10.1142/S0192415X09007260.