PMID- 19656198
OWN - NLM
STAT- MEDLINE
DCOM- 20091214
LR  - 20191210
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 298
IP  - 2
DP  - 2009 Sep
TI  - A nested PCR approach for improved recovery of archaeal 16S rRNA gene fragments 
      from freshwater samples.
PG  - 193-8
LID - 10.1111/j.1574-6968.2009.01718.x [doi]
AB  - In a survey on the presence of archaea in a number of European lakes, it was 
      found that known archaeal primer sets for PCR were not suited for use in 
      freshwater environment, as some lack selectivity, while others were too 
      selective. A nested PCR was developed for denaturing gradient gel electrophoresis 
      (DGGE) with primer sets 21F-958R and Parch519f-Arch915r, respectively. After 
      sequencing of the DGGE bands obtained by this nested method, 93% of the sequences 
      were of archaeal origin. More diverse archaeal DGGE patterns were found as 
      compared with other PCR methods. The nested PCR-DGGE method presented here is 
      therefore a reliable tool to analyze the archaeal diversity in freshwater 
      habitats, revealing even more widespread diversity of the archaea.
FAU - Vissers, Elisabeth W
AU  - Vissers EW
AD  - Department of Microbial Wetland Ecology, Centre for Limnology, Netherlands 
      Institute of Ecology (NIOO-KNAW), Nieuwersluis, The Netherlands. 
      l.vissers@nioo.knaw.nl
FAU - Bodelier, Paul L E
AU  - Bodelier PL
FAU - Muyzer, Gerard
AU  - Muyzer G
FAU - Laanbroek, Hendrikus J
AU  - Laanbroek HJ
LA  - eng
SI  - GENBANK/FJ475241
SI  - GENBANK/FJ475242
SI  - GENBANK/FJ475243
SI  - GENBANK/FJ475244
SI  - GENBANK/FJ475245
SI  - GENBANK/FJ475246
SI  - GENBANK/FJ475247
SI  - GENBANK/FJ475248
SI  - GENBANK/FJ475249
SI  - GENBANK/FJ475250
SI  - GENBANK/FJ475251
SI  - GENBANK/FJ475252
SI  - GENBANK/FJ475253
SI  - GENBANK/FJ475254
SI  - GENBANK/FJ475255
SI  - GENBANK/FJ475256
SI  - GENBANK/FJ475257
SI  - GENBANK/FJ475258
SI  - GENBANK/FJ475259
SI  - GENBANK/FJ475260
SI  - GENBANK/FJ475261
SI  - GENBANK/FJ475262
SI  - GENBANK/FJ475263
SI  - GENBANK/FJ475264
SI  - GENBANK/FJ475265
SI  - GENBANK/FJ475266
SI  - GENBANK/FJ475267
SI  - GENBANK/FJ475268
SI  - GENBANK/FJ475269
SI  - GENBANK/FJ475270
SI  - GENBANK/FJ475271
SI  - GENBANK/FJ475272
SI  - GENBANK/FJ475273
SI  - GENBANK/FJ475274
SI  - GENBANK/FJ475275
SI  - GENBANK/FJ475276
SI  - GENBANK/FJ475277
SI  - GENBANK/FJ475278
SI  - GENBANK/FJ475279
SI  - GENBANK/FJ475280
SI  - GENBANK/FJ475281
SI  - GENBANK/FJ475282
SI  - GENBANK/FJ475283
SI  - GENBANK/FJ475284
SI  - GENBANK/FJ475285
SI  - GENBANK/FJ475286
SI  - GENBANK/FJ475287
SI  - GENBANK/FJ475288
SI  - GENBANK/FJ475289
SI  - GENBANK/FJ475290
SI  - GENBANK/FJ475291
SI  - GENBANK/FJ475292
SI  - GENBANK/FJ475293
SI  - GENBANK/FJ475294
SI  - GENBANK/FJ475295
SI  - GENBANK/FJ475296
SI  - GENBANK/FJ475297
SI  - GENBANK/FJ475298
SI  - GENBANK/FJ475299
SI  - GENBANK/FJ475300
SI  - GENBANK/FJ475301
SI  - GENBANK/FJ475302
SI  - GENBANK/FJ475303
SI  - GENBANK/FJ475304
SI  - GENBANK/FJ475305
SI  - GENBANK/FJ475306
SI  - GENBANK/FJ475307
SI  - GENBANK/FJ475308
SI  - GENBANK/FJ475309
SI  - GENBANK/FJ475310
SI  - GENBANK/FJ475311
SI  - GENBANK/FJ475312
SI  - GENBANK/FJ475313
SI  - GENBANK/FJ475314
SI  - GENBANK/FJ475315
SI  - GENBANK/FJ475316
SI  - GENBANK/FJ475317
SI  - GENBANK/FJ475318
SI  - GENBANK/FJ475319
SI  - GENBANK/FJ475320
SI  - GENBANK/FJ475321
SI  - GENBANK/FJ475322
SI  - GENBANK/FJ475323
SI  - GENBANK/FJ475324
SI  - GENBANK/FJ475325
SI  - GENBANK/FJ475326
SI  - GENBANK/FJ475327
SI  - GENBANK/FJ475328
SI  - GENBANK/FJ475329
SI  - GENBANK/FJ475330
SI  - GENBANK/FJ475331
SI  - GENBANK/FJ475332
SI  - GENBANK/FJ475333
SI  - GENBANK/FJ475334
SI  - GENBANK/FJ475335
SI  - GENBANK/FJ475336
SI  - GENBANK/FJ475337
SI  - GENBANK/FJ475338
SI  - GENBANK/FJ475339
SI  - GENBANK/FJ475340
SI  - GENBANK/FJ475341
SI  - GENBANK/FJ475342
SI  - GENBANK/FJ475343
SI  - GENBANK/FJ475344
SI  - GENBANK/FJ475345
SI  - GENBANK/FJ475346
SI  - GENBANK/FJ475347
SI  - GENBANK/FJ475348
SI  - GENBANK/FJ475349
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090710
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Archaea/genetics/*isolation & purification
MH  - Cluster Analysis
MH  - DNA Primers/genetics
MH  - DNA, Archaeal/genetics/*isolation & purification
MH  - DNA, Ribosomal/genetics/*isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fresh Water/*microbiology
MH  - Molecular Sequence Data
MH  - Nucleic Acid Denaturation
MH  - Phylogeny
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Sequence Analysis, DNA
EDAT- 2009/08/07 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/08/07 09:00
PHST- 2009/08/07 09:00 [entrez]
PHST- 2009/08/07 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - FML1718 [pii]
AID - 10.1111/j.1574-6968.2009.01718.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2009 Sep;298(2):193-8. doi: 
      10.1111/j.1574-6968.2009.01718.x. Epub 2009 Jul 10.