PMID- 19657353
OWN - NLM
STAT- MEDLINE
DCOM- 20100128
LR  - 20181201
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Linking)
VI  - 130
IP  - 1
DP  - 2010 Jan
TI  - Measurement of CD4+ and CD8+ T-lymphocyte cytokine secretion and gene expression 
      changes in p-phenylenediamine allergic patients and tolerant individuals.
PG  - 161-74
LID - 10.1038/jid.2009.187 [doi]
AB  - Factors predisposing to individual susceptibility to contact allergic dermatitis 
      are ill defined. This study was designed to characterize the response of allergic 
      and tolerant individuals' T-lymphocytes after exposure to p-phenylenediamine 
      (PPD). Peripheral blood mononuclear cells (PBMCs) from allergic patients 
      proliferated when treated with PPD and Bandrowski's base (BB) and secreted 
      IL-1alpha, -1beta, -4, -5, -6, -8, -10, and -13; IFN-gamma; tumor necrosis 
      factor-alpha; MIP-1alpha/beta; MCP-1 (monocyte chemotactic protein-1); and 
      RANTES. PBMCs from tolerant individuals were stimulated to proliferate only with 
      BB, and they secreted significantly lower levels of Th2 cytokines. Principal 
      component analysis showed that genes are differentially expressed between the 
      patient groups. A network-based analysis of microarray data showed upregulation 
      of T helper type 2 (Th2) gene pathways, including IL-9, in allergic patients, but 
      a regulatory gene profile in tolerant individuals. Real-time PCR confirmed the 
      observed increase in Th2 cytokine gene transcription in allergic patients. 
      Purified CD4+ and CD8+ T cells from allergic patients were stimulated to 
      proliferate and secrete Th2 cytokines following antigen exposure. Only CD4+ T 
      cells from tolerant individuals were stimulated by BB, and levels of Th2 
      cytokines were 80% lower. The nature of the antigenic determinant stimulating 
      PBMCs and levels of Th2 cytokines, including IL-9, was confirmed in a validation 
      cohort. These studies show increased activity of Th2 cytokines in CD4+ and CD8+ T 
      cells from individuals with allergic contact dermatitis.
FAU - Coulter, Eve M
AU  - Coulter EM
AD  - Department of Pharmacology, MRC Centre for Drug Safety Science, University of 
      Liverpool, Liverpool, UK.
FAU - Jenkinson, Claire
AU  - Jenkinson C
FAU - Farrell, John
AU  - Farrell J
FAU - Lavergne, Sidonie N
AU  - Lavergne SN
FAU - Pease, Camilla
AU  - Pease C
FAU - White, Andrew
AU  - White A
FAU - Aleksic, Maja
AU  - Aleksic M
FAU - Basketter, David
AU  - Basketter D
FAU - Williams, Dominic P
AU  - Williams DP
FAU - King, Clodagh
AU  - King C
FAU - Pirmohamed, Munir
AU  - Pirmohamed M
FAU - Park, B Kevin
AU  - Park BK
FAU - Naisbitt, Dean J
AU  - Naisbitt DJ
LA  - eng
GR  - G0700654/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Cytokines)
RN  - 0 (IL5 protein, human)
RN  - 0 (IL9 protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-5)
RN  - 0 (Interleukin-9)
RN  - 0 (Phenylenediamines)
RN  - 0 (Tuberculin)
RN  - 20048-27-5 (Bandrowski's base)
RN  - U770QIT64J (4-phenylenediamine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - Cell Division/immunology
MH  - Cytokines/genetics/*metabolism
MH  - Dermatitis, Allergic Contact/genetics/*immunology/metabolism
MH  - Female
MH  - Gene Expression/immunology
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immune Tolerance/*immunology
MH  - Interleukin-13/genetics/metabolism
MH  - Interleukin-5/genetics/metabolism
MH  - Interleukin-9/genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Phenylenediamines/adverse effects/immunology
MH  - Skin Tests
MH  - Th2 Cells/immunology/metabolism
MH  - Tuberculin/adverse effects/immunology
MH  - Young Adult
EDAT- 2009/08/07 09:00
MHDA- 2010/01/29 06:00
CRDT- 2009/08/07 09:00
PHST- 2009/08/07 09:00 [entrez]
PHST- 2009/08/07 09:00 [pubmed]
PHST- 2010/01/29 06:00 [medline]
AID - S0022-202X(15)34540-1 [pii]
AID - 10.1038/jid.2009.187 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2010 Jan;130(1):161-74. doi: 10.1038/jid.2009.187.