PMID- 19658087
OWN - NLM
STAT- MEDLINE
DCOM- 20100302
LR  - 20091120
IS  - 1932-5231 (Electronic)
IS  - 1932-5223 (Linking)
VI  - 311
IP  - 10
DP  - 2009 Dec 1
TI  - Nucleolar binding of an anti-NMDA receptor antibody in hydra: a non-canonical 
      role for an NMDA receptor protein?
PG  - 763-75
LID - 10.1002/jez.563 [doi]
AB  - Two emerging concepts in cell biology are the back-and-forth trafficking of 
      receptor proteins and nuclear transcription factors between the nucleus and the 
      cell membrane, and the alternative splicing of messenger RNA to produce similar 
      proteins targeted to different cell sites. Recent evidence suggests that the 
      nucleolus is a dynamic structure whose components may be involved in both types 
      of trafficking. In the nervous system of higher animals, the N-methyl-D-aspartate 
      (NMDA)-specific glutamate receptor has various roles in development and cell 
      communication. It is involved in learning, memory, axonal guidance and nerve 
      regeneration. We have reported earlier that the NR1 subunit of the NMDA receptor 
      is present in the cell periphery and the nucleus of stem cells, neurons and 
      epitheliomuscular cells of the early-evolved cnidarian, Hydra vulgaris 
      (Scappaticci et al., 2004. Cell Tissue Res 316:263-270); it is involved in 
      coordinating hydra's neuroeffector systems (Kass-Simon and Scappaticci, 2003. 
      Hydrobiologia 530/531:67-71; Pierobon et al., 2004. Eur J Neurosci 20:2598-2604; 
      Scappaticci and Kass-Simon, 2008. Comp Biochem Physiol A 150:415-422; Kay and 
      Kass-Simon, 2009. Bio Bull 216:113-129). Here we report immunocytochemical 
      experiments, using a mouse monoclonal antibody raised against the mammalian NR1 
      receptor subunit, and an in silico genomic and gene expression analysis 
      identifying the homologues in hydra of mammalian NR1 and fibrillarin (FBL) genes, 
      and their expressed proteins. The experiments reveal that the NR1 antibody 
      specifically labels the nucleoli of large and small interstitial cells (stem 
      cells), nematoblasts, neuroblasts, and epitheliomusclar cells; antibody labeling 
      of the nucleolar marker, FBL, confirms the nucleolar localization of NR1 antibody 
      labeling. Genomic analysis reveals that NR1 and FBL genes are conserved in hydra, 
      and suggests that there are at least two NR1 splice variants, one of which 
      contains both nuclear and nucleolar targeting signals. The finding that an NR1 
      receptor subunit (or a portion of it) appears in nucleoli of hydra cells is 
      unique, and has not been reported for any other organism. Its presence in 
      nucleoli of hydra may signal the existence of a yet-undescribed shuttle mechanism 
      between the cell surface and the nucleous, or the alternative deployment of NR1 
      splice variants to different cell sites.
FAU - Kass-Simon, Gabriele
AU  - Kass-Simon G
AD  - Department of Biological Sciences, University of Rhode Island, Morrill Hall, 
      Kingston, RI 02881, USA. kass.simon@uri.edu
FAU - Zompa, Michael A
AU  - Zompa MA
FAU - Scappaticci, Albert A
AU  - Scappaticci AA
FAU - Zackroff, Robert V
AU  - Zackroff RV
FAU - Hufnagel, Linda A
AU  - Hufnagel LA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Exp Zool A Ecol Genet Physiol
JT  - Journal of experimental zoology. Part A, Ecological genetics and physiology
JID - 101297745
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (fibrillarin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Cell Nucleolus/*metabolism
MH  - Chromosomal Proteins, Non-Histone/genetics/*metabolism
MH  - Gene Expression Regulation/physiology
MH  - Hydra/*metabolism
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Sequence Homology
MH  - Stem Cells/metabolism
MH  - Tissue Distribution
EDAT- 2009/08/07 09:00
MHDA- 2010/03/03 06:00
CRDT- 2009/08/07 09:00
PHST- 2009/08/07 09:00 [entrez]
PHST- 2009/08/07 09:00 [pubmed]
PHST- 2010/03/03 06:00 [medline]
AID - 10.1002/jez.563 [doi]
PST - ppublish
SO  - J Exp Zool A Ecol Genet Physiol. 2009 Dec 1;311(10):763-75. doi: 10.1002/jez.563.