PMID- 1965884
OWN - NLM
STAT- MEDLINE
DCOM- 19910801
LR  - 20181113
IS  - 1044-2030 (Print)
IS  - 1044-2030 (Linking)
VI  - 1
IP  - 7
DP  - 1990 Jun
TI  - The order of processing events in mouse mammary tumor virus envelope protein 
      maturation: implications for the location of the glucocorticoid-regulated step.
PG  - 531-41
AB  - Treatment of the W7MG1 mouse T lymphoma cell line with glucocorticoid stimulates 
      directly or indirectly two observable steps in the processing of mouse mammary 
      tumor virus (MMTV) envelope glycoprotein precursor Pr74: cleavage of Pr74 to 
      yield the mature glycoprotein products gp52 and gp33, and processing of the 
      N-linked oligosaccharides to endoglycosidase H (endo H)-resistant forms found on 
      the mature products but not on the precursor. Therefore, the primary 
      hormone-regulated event in this pathway must occur at or before the point where 
      MMTV envelope proteins become endo H resistant. Pulse-chase analyses identified a 
      novel endo H-resistant 80-kDa species (designated gp80) as a processing 
      intermediate. Therefore, in contrast to conclusions drawn for the envelope 
      proteins of several other retroviruses, proteolytic cleavage of MMTV envelope 
      proteins occurs after acquisition of endo H resistance. Also, proteolytic 
      cleavage cannot be the primary hormone-regulated step. Second, inhibition of 
      mannosidase II by the drug swainsonine did not prevent Pr74 from being 
      proteolytically processed, thus demonstrating that conversion of oligosaccharide 
      chains from endo H-sensitive to -resistant forms was not a prerequisite for 
      proteolytic cleavage. Therefore, the requisite hormone-regulated event in MMTV 
      glycoprotein processing must precede both acquisition of endo H resistance and 
      proteolytic cleavage. This places the regulated event in the endoplasmic 
      reticulum or early Golgi.
FAU - Corey, J L
AU  - Corey JL
AD  - Department of Pathology, University of Southern California, Los Angeles 90033.
FAU - Stallcup, M R
AU  - Stallcup MR
LA  - eng
GR  - AG00093/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Regul
JT  - Cell regulation
JID - 9005331
RN  - 0 (Alkaloids)
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (Glucocorticoids)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 52 antigen, Mouse mammary tumor virus)
RN  - EC 3.2.1.- (Mannosidases)
RN  - EC 3.2.1.114 (mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase)
RN  - EC 3.4.- (Endopeptidases)
RN  - RSY4RK37KQ (Swainsonine)
SB  - IM
MH  - Alkaloids/pharmacology
MH  - Animals
MH  - Antigens, Viral, Tumor/metabolism
MH  - Endopeptidases/metabolism
MH  - Glucocorticoids/*metabolism
MH  - Kinetics
MH  - Mammary Tumor Virus, Mouse/*metabolism
MH  - Mannosidases/antagonists & inhibitors
MH  - Mice
MH  - *Protein Processing, Post-Translational
MH  - Swainsonine
MH  - Tumor Cells, Cultured
MH  - Viral Envelope Proteins/*metabolism
PMC - PMC361570
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
PMCR- 1990/06/01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PHST- 1990/06/01 00:00 [pmc-release]
AID - 10.1091/mbc.1.7.531 [doi]
PST - ppublish
SO  - Cell Regul. 1990 Jun;1(7):531-41. doi: 10.1091/mbc.1.7.531.