PMID- 19660577
OWN - NLM
STAT- MEDLINE
DCOM- 20100701
LR  - 20151119
IS  - 1879-3649 (Electronic)
IS  - 1537-1891 (Linking)
VI  - 51
IP  - 4
DP  - 2009 Oct
TI  - Hyperhomocysteinemia induced by feeding rats diets rich in DL-homocysteine 
      thiolactone promotes alterations on carotid reactivity independent of arterial 
      structure.
PG  - 291-8
LID - 10.1016/j.vph.2009.07.004 [doi]
AB  - We aimed to investigate whether hyperhomocysteinemia (HHcy) interferes on carotid 
      vascular reactivity, and how morphological and functional aspects are related. 
      With this purpose male Wistar rats received a solution of 
      dl-homocysteine-thiolactone (1g/kg body weight/day) in the drinking water for 4, 
      15 and 30 days. Lipid profile, carotid artery-morphology and -responsiveness to 
      acetylcholine, phenylephrine and endothelin-1 were analyzed. Similar increase on 
      homocysteine plasmatic levels occurred in rats treated for 4, 15 and 30 days. 
      High levels of serum cholesterol and triglycerides were observed after HHcy 30 
      days. Vascular reactivity experiments using standard muscle bath procedures 
      showed that HHcy induced a time-dependent reduction on 
      acetylcholine-induced-relaxation at 4, 15 and 30 days. HHcy enhanced the 
      contractile response of endothelium-intact, but not denuded carotid rings to 
      phenylephrine and endothelin-1, despite the treatment time. Morphometric analysis 
      showed that intimal/medial area ratio was enhanced only at 30 days of HHcy, 
      despite its reduced cell density. The major new finding of the present study is 
      that it establishes a time-course relationship for the events involved on 
      vascular effects associated with HHcy. We demonstrated that alterations on 
      vascular responsiveness precede alterations on arterial structure. Based on such 
      findings it is possible to suggest that vascular dysfunction occurs in early 
      stages while alterations on vessel morphology take place in latest stages of 
      HHcy.
FAU - de Andrade, Claudia Roberta
AU  - de Andrade CR
AD  - Department of Pharmacology, FMRP-USP, Ribeirao Preto, SP, Brazil.
FAU - Tirapelli, Carlos Renato
AU  - Tirapelli CR
FAU - Haddad, Renato
AU  - Haddad R
FAU - Eberlin, Marcos N
AU  - Eberlin MN
FAU - Ramalho, Leandra N Z
AU  - Ramalho LN
FAU - Iyomasa, Mamie M
AU  - Iyomasa MM
FAU - Uyemura, Sergio A
AU  - Uyemura SA
FAU - de Oliveira, Ana Maria
AU  - de Oliveira AM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090804
PL  - United States
TA  - Vascul Pharmacol
JT  - Vascular pharmacology
JID - 101130615
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - D5H88XF24X (homocysteine thiolactone)
SB  - IM
MH  - Animals
MH  - Carotid Arteries/drug effects/pathology/*physiology
MH  - Feeding Methods
MH  - Homocysteine/administration & dosage/*analogs & derivatives/toxicity
MH  - Hyperhomocysteinemia/chemically induced/*physiopathology
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Vasoconstriction/drug effects/*physiology
MH  - Vasodilation/drug effects/*physiology
EDAT- 2009/08/08 09:00
MHDA- 2010/07/02 06:00
CRDT- 2009/08/08 09:00
PHST- 2009/02/07 00:00 [received]
PHST- 2009/05/17 00:00 [revised]
PHST- 2009/07/21 00:00 [accepted]
PHST- 2009/08/08 09:00 [entrez]
PHST- 2009/08/08 09:00 [pubmed]
PHST- 2010/07/02 06:00 [medline]
AID - S1537-1891(09)00093-7 [pii]
AID - 10.1016/j.vph.2009.07.004 [doi]
PST - ppublish
SO  - Vascul Pharmacol. 2009 Oct;51(4):291-8. doi: 10.1016/j.vph.2009.07.004. Epub 2009 
      Aug 4.