PMID- 19663511
OWN - NLM
STAT- MEDLINE
DCOM- 20091224
LR  - 20090904
IS  - 1535-3893 (Print)
IS  - 1535-3893 (Linking)
VI  - 8
IP  - 9
DP  - 2009 Sep
TI  - Outer membrane proteome and antigens of Tannerella forsythia.
PG  - 4279-92
LID - 10.1021/pr900372c [doi]
AB  - Tannerella forsythia is a Gram-negative, anaerobic, fusiform bacterium implicated 
      as a periodontal pathogen. With use of 2D PAGE, SDS PAGE, and LC-MALDI-TOF/TOF 
      MS, 221 proteins of T. forsythia outer membrane preparations were identified, of 
      which 197 were predicted to be localized to the cell envelope. Fifty-six proteins 
      were reproducibly mapped by 2D PAGE and included several highly abundant proteins 
      in the MW range 140-250 kDa that exhibited C-terminal sequence similarity to the 
      CTD family of Porphyromonas gingivalis. Two-dimensional Western blot analyses 
      revealed that these CTD family proteins together with several other outer 
      membrane proteins were antigenic. The CTD family proteins exhibited a higher than 
      expected MW, and were strongly reactive with the fluorescent glycoprotein stain, 
      ProQ Emerald. This group included BspA and surface layer proteins A and B. 
      TonB-dependent receptors (TDRs) (46) were identified together with 28 putative 
      lipoproteins whose genes are immediately downstream of a TDR gene. The major 
      OmpA-like protein was found to be TF1331. Uniquely, it was found to exist as a 
      homodimer held together by up to three disulfide bridges as demonstrated by MS/MS 
      of a tryptic peptide derived from unreduced TF1331.
FAU - Veith, Paul D
AU  - Veith PD
AD  - Cooperative Research Centre for Oral Health Science, Melbourne Dental School, 
      Bio21 Institute, The University of Melbourne, Melbourne, Victoria, Australia.
FAU - O'Brien-Simpson, Neil M
AU  - O'Brien-Simpson NM
FAU - Tan, Yan
AU  - Tan Y
FAU - Djatmiko, Deasy C
AU  - Djatmiko DC
FAU - Dashper, Stuart G
AU  - Dashper SG
FAU - Reynolds, Eric C
AU  - Reynolds EC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Lipoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (tonB protein, Bacteria)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Bacterial/*analysis
MH  - Bacterial Outer Membrane Proteins/*analysis
MH  - Bacterial Proteins/analysis
MH  - Bacteroides/*chemistry/immunology
MH  - Blotting, Western
MH  - Chromatography, Liquid
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Lipoproteins/analysis
MH  - Membrane Proteins/analysis
MH  - Molecular Sequence Data
MH  - Proteomics/*methods
MH  - Sequence Alignment
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2009/08/12 09:00
MHDA- 2009/12/25 06:00
CRDT- 2009/08/12 09:00
PHST- 2009/08/12 09:00 [entrez]
PHST- 2009/08/12 09:00 [pubmed]
PHST- 2009/12/25 06:00 [medline]
AID - 10.1021/pr900372c [doi]
PST - ppublish
SO  - J Proteome Res. 2009 Sep;8(9):4279-92. doi: 10.1021/pr900372c.