PMID- 19664603
OWN - NLM
STAT- MEDLINE
DCOM- 20090921
LR  - 20191210
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 388
IP  - 1
DP  - 2009 Oct 9
TI  - Lycopene inhibits PDGF-BB-induced retinal pigment epithelial cell migration by 
      suppression of PI3K/Akt and MAPK pathways.
PG  - 172-6
LID - 10.1016/j.bbrc.2009.07.155 [doi]
AB  - Retinal pigment epithelial (RPE) cells play a dominant role in the development of 
      proliferative vitreoretinopathy (PVR), which is the leading cause of failure in 
      retinal reattachment surgery. Several studies have shown that platelet-derived 
      growth factor (PDGF) exhibits chemotaxis and proliferation effects on RPE cells 
      in PVR. In this study, the inhibitory effect of lycopene on PDGF-BB-induced 
      ARPE19 cell migration is examined. In electric cell-substrate impedance sensing 
      (ECIS) and Transwell migration assays, significant suppression of PDGF-BB-induced 
      ARPE19 cell migration by lycopene is observed. Cell viability assays show no 
      cytotoxicity of lycopene on RPE cells. Lycopene shows no effect on ARPE19 cell 
      adhesion and is found to inhibit PDGF-BB-induced tyrosine phosphorylation and the 
      underlying signaling pathways of PI3K, Akt, ERK and p38 activation. However, 
      PDGF-BB and lycopene show no effects on JNK activation. Taken together, our 
      results demonstrate that lycopene inhibits PDGF-BB-induced ARPE19 cell migration 
      through inhibition of PI3K/Akt, ERK and p38 activation.
FAU - Chan, Chi-Ming
AU  - Chan CM
AD  - Fu Jen Catholic University, Taipei Hsien, Taiwan, ROC.
FAU - Fang, Jia-You
AU  - Fang JY
FAU - Lin, Hsin-Huang
AU  - Lin HH
FAU - Yang, Chi-Yea
AU  - Yang CY
FAU - Hung, Chi-Feng
AU  - Hung CF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090805
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antioxidants)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 1B56C968OA (Becaplermin)
RN  - 36-88-4 (Carotenoids)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - SB0N2N0WV6 (Lycopene)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Becaplermin
MH  - Carotenoids/*pharmacology
MH  - Cell Line
MH  - Cell Movement/*drug effects
MH  - Humans
MH  - Lycopene
MH  - Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation/drug effects
MH  - Platelet-Derived Growth Factor/antagonists & inhibitors/pharmacology/*physiology
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors
MH  - Proto-Oncogene Proteins c-sis
MH  - Retinal Pigment Epithelium/*drug effects/physiology
EDAT- 2009/08/12 09:00
MHDA- 2009/09/22 06:00
CRDT- 2009/08/12 09:00
PHST- 2009/07/11 00:00 [received]
PHST- 2009/07/31 00:00 [accepted]
PHST- 2009/08/12 09:00 [entrez]
PHST- 2009/08/12 09:00 [pubmed]
PHST- 2009/09/22 06:00 [medline]
AID - S0006-291X(09)01554-X [pii]
AID - 10.1016/j.bbrc.2009.07.155 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Oct 9;388(1):172-6. doi: 
      10.1016/j.bbrc.2009.07.155. Epub 2009 Aug 5.