PMID- 19665607
OWN - NLM
STAT- MEDLINE
DCOM- 20091105
LR  - 20090928
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 27
IP  - 43
DP  - 2009 Oct 9
TI  - Evaluation of murine bone marrow-derived dendritic cells loaded with inactivated 
      virus as a vaccine against Japanese encephalitis virus.
PG  - 6004-10
LID - 10.1016/j.vaccine.2009.07.078 [doi]
AB  - Japanese encephalitis (JE) is a serious infectious disease in southern and 
      eastern Asia. Design and development of safer and more efficacious vaccines 
      against Japanese encephalitis virus (JEV) is a high-priority target in the world. 
      Dendritic cells (DCs) are the most potent professional antigen-presenting cells 
      (APCs) playing a central and unique role in the generation of primary T-cell 
      responses, and are considered attractive "live adjuvants" for vaccination and 
      immunotherapy against cancer and infectious diseases. In this study, mouse bone 
      marrow-derived dendritic cells (bmDCs were generated and stimulated with 
      inactivated JEV in vitro. BALB/c mice were immunized with stimulated bmDCs and 
      then challenged with JEV wild-type strain. The neutralization antibody, 
      interferon gamma (IFN-gamma), tumor necrosis factors alpha (TNF-alpha) or 
      interleukin-6 (IL-6), and virus-specific CD8+ cytotoxic T-lymphocyte (CTL) 
      levels, as well as survival rates, were analyzed and compared with inactivated 
      vaccine and DCs control groups. The results demonstrated that intravenous (i.v.) 
      injection of 2 x 10(5) JEV-pulsed bmDCs into each mouse produced notable levels 
      of JEV-specific neutralizing antibodies and higher levels of CD8+ CTL, IFN-gamma 
      and TNF-alpha compared with JEV-inactivated vaccine. Furthermore, stimulated 
      bmDCs could elicit a highly protective efficacy (90%) against JEV challenge. It 
      suggests that stimulated bmDCs can be considered as an attractive "live adjuvant" 
      for vaccination against JEV infection.
FAU - Li, Yaoming
AU  - Li Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan, Hubei, 430070, PR China.
FAU - Xu, Min
AU  - Xu M
FAU - Chen, Long
AU  - Chen L
FAU - Zhu, Jinghui
AU  - Zhu J
FAU - Ye, Jing
AU  - Ye J
FAU - Liu, Xueqin
AU  - Liu X
FAU - Sun, Yanfang
AU  - Sun Y
FAU - Chen, Huanchun
AU  - Chen H
FAU - Cao, Shengbo
AU  - Cao S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090807
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Interleukin-6)
RN  - 0 (Japanese Encephalitis Vaccines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/*immunology
MH  - Encephalitis Virus, Japanese/*immunology
MH  - Encephalitis, Japanese/immunology/*prevention & control
MH  - Female
MH  - Immunity, Cellular
MH  - Interferon-gamma
MH  - Interleukin-6/immunology
MH  - Japanese Encephalitis Vaccines/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neutralization Tests
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Tumor Necrosis Factor-alpha/immunology
EDAT- 2009/08/12 09:00
MHDA- 2009/11/06 06:00
CRDT- 2009/08/12 09:00
PHST- 2009/04/05 00:00 [received]
PHST- 2009/07/06 00:00 [revised]
PHST- 2009/07/22 00:00 [accepted]
PHST- 2009/08/12 09:00 [entrez]
PHST- 2009/08/12 09:00 [pubmed]
PHST- 2009/11/06 06:00 [medline]
AID - S0264-410X(09)01105-0 [pii]
AID - 10.1016/j.vaccine.2009.07.078 [doi]
PST - ppublish
SO  - Vaccine. 2009 Oct 9;27(43):6004-10. doi: 10.1016/j.vaccine.2009.07.078. Epub 2009 
      Aug 7.