PMID- 19665774
OWN - NLM
STAT- MEDLINE
DCOM- 20091113
LR  - 20240109
IS  - 1097-6825 (Electronic)
IS  - 0091-6749 (Linking)
VI  - 124
IP  - 4
DP  - 2009 Oct
TI  - Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a 
      Mediterranean allergic population.
PG  - 771-8.e5
LID - 10.1016/j.jaci.2009.06.008 [doi]
AB  - BACKGROUND: Nonspecific lipid transfer proteins (LTPs) represent potent pollen 
      and food allergens. However, the allergenic properties of peanut LTP have not 
      been studied. OBJECTIVE: To identify LTP in peanut extract using sera from 
      subjects with peanut allergy and Pru p 3-sensitized subjects from Southern 
      Europe, clone and express this protein, and obtain information on the importance 
      as allergen for these selected patients. METHODS: Peanut LTP (Ara h 9) was cloned 
      and sequenced by using a combination of bioinformatic and molecular biology tools 
      (PCR, immunoblotting, Basic Local Alignment Search Tool [BLAST] searches). The 
      immunologic properties of Ara h 9, Ara h 1, Ara h 2, and Ara h 3 were studied by 
      using sera from subjects with peanut and peach allergy from Italy by 
      immunoblotting and allergen microarray technology. RESULTS: Two Ara h 9 
      isoforms-Ara h 9.01 and Ara h 9.02-were cloned and expressed. Ara h 9 represented 
      a minor allergen for subjects with peanut allergy. However, including Ara h 9 as 
      single component for serologic detection of sensitization to peanut by 
      component-resolved diagnosis seems crucial, because the frequency of 
      sensitization to the classic major peanut allergens Ara h 1, Ara h 2, and Ara h 3 
      was low in these patients from Southern Europe. CONCLUSION: Ara h 9 is a new 
      member of the LTP allergen family that seems to play an important role in peanut 
      allergy for patients from the Mediterranean area.
FAU - Krause, Susanne
AU  - Krause S
AD  - Research Center Borstel, Borstel, Germany.
FAU - Reese, Gerald
AU  - Reese G
FAU - Randow, Stefanie
AU  - Randow S
FAU - Zennaro, Danila
AU  - Zennaro D
FAU - Quaratino, Donato
AU  - Quaratino D
FAU - Palazzo, Paola
AU  - Palazzo P
FAU - Ciardiello, Maria Antonietta
AU  - Ciardiello MA
FAU - Petersen, Arnd
AU  - Petersen A
FAU - Becker, Wolf-Meinhard
AU  - Becker WM
FAU - Mari, Adriano
AU  - Mari A
LA  - eng
PT  - Journal Article
DEP - 20090808
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (2S Albumins, Plant)
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Ara h 1 protein, Arachis hypogaea)
RN  - 0 (Ara h 2 allergen, Arachis hypogaea)
RN  - 0 (Ara h 9 allergen, peanut)
RN  - 0 (Carrier Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Seed Storage Proteins)
RN  - 0 (allergen Ara h3)
RN  - 0 (lipid transfer protein)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - 2S Albumins, Plant/immunology/metabolism
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Allergens/*immunology/metabolism
MH  - Antigens, Plant/immunology/metabolism
MH  - Arachis/*immunology
MH  - Carrier Proteins/*immunology/isolation & purification/metabolism
MH  - Child
MH  - Cross Reactions/immunology
MH  - Female
MH  - Glycoproteins/*immunology/metabolism
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Membrane Proteins
MH  - Middle Aged
MH  - Peanut Hypersensitivity/*immunology/metabolism
MH  - Plant Proteins/*immunology/metabolism
MH  - Protein Isoforms/immunology/metabolism
MH  - Seed Storage Proteins/immunology/metabolism
MH  - Young Adult
EDAT- 2009/08/12 09:00
MHDA- 2009/11/17 06:00
CRDT- 2009/08/12 09:00
PHST- 2009/02/13 00:00 [received]
PHST- 2009/05/29 00:00 [revised]
PHST- 2009/06/02 00:00 [accepted]
PHST- 2009/08/12 09:00 [entrez]
PHST- 2009/08/12 09:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
AID - S0091-6749(09)00875-6 [pii]
AID - 10.1016/j.jaci.2009.06.008 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2009 Oct;124(4):771-8.e5. doi: 
      10.1016/j.jaci.2009.06.008. Epub 2009 Aug 8.