PMID- 19666020
OWN - NLM
STAT- MEDLINE
DCOM- 20091130
LR  - 20220408
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 87
IP  - 3
DP  - 2009 Dec
TI  - Hematopoietic stem cell engraftment by early-stage in utero transplantation in a 
      mouse model.
PG  - 173-7
LID - 10.1016/j.yexmp.2009.07.009 [doi]
AB  - A novel intrauterine transplantation (IUT) approach was developed to improve the 
      efficiency of engraftment of hematopoietic stem cells (HSCs). HSCs with a green 
      fluorescent protein (GFP) reporter gene were transplanted in utero on days 12.5, 
      13.5 and 14.5 post coitum (p.c.). The degree of chimerism of donor cells in 
      recipient newborn mice was examined using fluorescent microscopy, polymerase 
      chain reaction (PCR), fluorescence-activated cell sorting (FACS), and 
      fluorescence in situ hybridization (FISH) analyses. Microscopic examination 
      revealed the presence of green fluorescent signal in the peripheral blood of the 
      chimeric mice. The highest survival rate (47%) as well as the highest chimerism 
      rate (73%) were achieved by our new approach in the newborn mice that were 
      subjected to in utero transplantation (IUT) on day 12.5 p.c. (E12.5) compared to 
      the conventional IUT method. FACS analysis indicated that 1.55+/-1.10% of 
      peripheral blood cells from the newborn mice were GFP-positive donor cells. FISH 
      showed that cells containing the donor-specific GFP sequence were present in the 
      bone marrow (BM) of the chimeric mice. Thus, the efficiency of chimera production 
      with this new method of IUT was significantly improved over the existing IUT 
      techniques and instruments.
FAU - Chen, Xin
AU  - Chen X
AD  - Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 
      Jiao Tong University School of Medicine, Shanghai, China.
FAU - Gong, Xiu-li
AU  - Gong XL
FAU - Katsumata, Makoto
AU  - Katsumata M
FAU - Zeng, Yi-tao
AU  - Zeng YT
FAU - Huang, Shu-zhen
AU  - Huang SZ
FAU - Zeng, Fanyi
AU  - Zeng F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090807
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Cell Separation
MH  - Cell Survival
MH  - Chimera
MH  - *Chimerism
MH  - Female
MH  - Flow Cytometry
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins/blood/genetics
MH  - Hematopoietic Stem Cell Transplantation/instrumentation/*methods
MH  - Hematopoietic Stem Cells/cytology/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Microscopy, Fluorescence
MH  - Models, Animal
MH  - *Uterus
EDAT- 2009/08/12 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/08/12 09:00
PHST- 2009/07/09 00:00 [received]
PHST- 2009/07/30 00:00 [accepted]
PHST- 2009/08/12 09:00 [entrez]
PHST- 2009/08/12 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0014-4800(09)00095-1 [pii]
AID - 10.1016/j.yexmp.2009.07.009 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2009 Dec;87(3):173-7. doi: 10.1016/j.yexmp.2009.07.009. Epub 2009 
      Aug 7.