PMID- 1966893
OWN - NLM
STAT- MEDLINE
DCOM- 19920507
LR  - 20181113
IS  - 1044-2030 (Print)
IS  - 1044-2030 (Linking)
VI  - 1
IP  - 13
DP  - 1990 Dec
TI  - Tyrosine phosphorylation of the gap junction protein connexin43 is required for 
      the pp60v-src-induced inhibition of communication.
PG  - 989-1002
AB  - Gap junction communication in some cells has been shown to be inhibited by 
      pp60v-src, a protein tyrosine kinase encoded by the viral oncogene v-src. The gap 
      junction protein connexin43 (Cx43) has been shown to be phosphorylated on serine 
      in the absence of pp60v-src and on both serine and tyrosine in cells expressing 
      pp60v-src. However, it is not known if the effect of v-src expression on 
      communication results directly from tyrosine phosphorylation of the Cx43 or 
      indirectly, for example, by activation of other second-messenger systems. In 
      addition, the effect of v-src expression on communication based on other 
      connexins has not been examined. We have used a functional expression system 
      consisting of paired Xenopus oocytes to examine the effect of v-src expression on 
      the regulation of communication by gap junctions comprised of different 
      connexins. Expression of pp60v-src completely blocked the communication induced 
      by Cx43 but had only a modest effect on communication induced by connexin32 
      (Cx32). Phosphoamino acid analysis showed that pp60v-src induced tyrosine 
      phosphorylation of Cx43, but not Cx32. A mutation replacing tyrosine 265 of Cx43 
      with phenylalanine abolished both the inhibition of communication and the 
      tyrosine phosphorylation induced by pp60v-src without affecting the ability of 
      this protein to form gap junctions. These data show that the effect of pp60v-src 
      on gap junctional communication is connexin specific and that the inhibition of 
      Cx43-mediated junctional communication by pp60v-src requires tyrosine 
      phosphorylation of Cx43.
FAU - Swenson, K I
AU  - Swenson KI
AD  - Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, 
      Massachusetts.
FAU - Piwnica-Worms, H
AU  - Piwnica-Worms H
FAU - McNamee, H
AU  - McNamee H
FAU - Paul, D L
AU  - Paul DL
LA  - eng
GR  - CA-50767/CA/NCI NIH HHS/United States
GR  - GM-18974/GM/NIGMS NIH HHS/United States
GR  - GM-37751/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Regul
JT  - Cell regulation
JID - 9005331
RN  - 0 (Connexins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 42HK56048U (Tyrosine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.10.2 (Oncogene Protein pp60(v-src))
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Communication/*physiology
MH  - Cell Division/physiology
MH  - Connexins
MH  - Gene Expression/physiology
MH  - Membrane Proteins/*metabolism
MH  - Microinjections
MH  - Molecular Sequence Data
MH  - Oncogene Protein pp60(v-src)/*metabolism
MH  - Oocytes/*metabolism
MH  - Phosphorylation
MH  - RNA, Messenger/metabolism
MH  - Serine/metabolism
MH  - Tyrosine/*metabolism
MH  - Xenopus
PMC - PMC361697
EDAT- 1990/12/01 00:00
MHDA- 1990/12/01 00:01
PMCR- 1990/12/01
CRDT- 1990/12/01 00:00
PHST- 1990/12/01 00:00 [pubmed]
PHST- 1990/12/01 00:01 [medline]
PHST- 1990/12/01 00:00 [entrez]
PHST- 1990/12/01 00:00 [pmc-release]
AID - 10.1091/mbc.1.13.989 [doi]
PST - ppublish
SO  - Cell Regul. 1990 Dec;1(13):989-1002. doi: 10.1091/mbc.1.13.989.