PMID- 19669206
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1868-9256 (Print)
IS  - 1865-5785 (Electronic)
IS  - 1865-5785 (Linking)
VI  - 1
IP  - 2
DP  - 2008 Sep
TI  - Primary mediastinal B-cell lymphoma: detection of BCL2 gene rearrangements by PCR 
      analysis and FISH.
PG  - 77-84
LID - 10.1007/s12308-008-0007-7 [doi]
AB  - Primary mediastinal large B-cell lymphoma (PMBCL) has a characteristic clinical 
      presentation, morphology, and immunophenotype, representing a clinically 
      favorable subgroup of diffuse large B-cell lymphoma (DLBCL). By gene expression 
      profiling (GEP), PMBCL shares features with classical Hodgkin lymphoma (cHL). Of 
      further interest, BCL6 gene mutations and BCL6 and/or MUM1 expression in a number 
      of PMBCLs have supported an activated B-cell (ABC) origin. Several studies, 
      including GEP, have failed to detect BCL2 gene rearrangements (GRs) in PMBCL. An 
      index case of t(14; 18)+ PMBCL prompted our study of the incidence of BCL2 GRs in 
      PMBCL by polymerase chain reaction (PCR)/fluorescence in situ hybridization 
      (FISH) analyses and its possible clinical impact. Twenty-five retrospectively 
      identified, well-defined PMBCLs (five with cytogenetics) from three institutions 
      were analyzed for a BCL2 GR by PCR/FISH analyses. The formalin-fixed, 
      paraffin-embedded tissue blocks of 24 available cases were also analyzed by BCL2 
      immunohistochemistry (IHC). Of the five with cytogenetics, two had a t(14; 18) 
      (q32; q21). Of the 25 analyzed by PCR, 2 had no amplifiable DNA (aDNA), including 
      1 t(14; 18)+ case. Of those with aDNA, two showed a BCL2 GR; by FISH analysis, 
      three demonstrated a BCL2 GR. BCL2 protein expression by IHC analysis was 
      variably detected in 21 out of 24 (strongly, uniformly expressed: 6, including 
      all with a t(14; 18) or a BCL2 gene rearrangement; moderately weakly expressed in 
      a subset of the malignant cells: 15). Available clinical follow-up of this BCL2+ 
      subset showed a similar course to the other PMBCL cases. Our results imply that a 
      subset of PMBCL [(4 out of 24 analyzed) in our series] may be of GC origin. A 
      larger study is necessary to determine any clinical significance.
FAU - Dunphy, Cherie H
AU  - Dunphy CH
AD  - Divisions of Hematopathology, University of North Carolina, Chapel Hill, NC, USA, 
      cdunphy@unch.unc.edu.
FAU - O'Malley, Dennis P
AU  - O'Malley DP
FAU - Cheng, Liang
AU  - Cheng L
FAU - Fodrie, Tina Y
AU  - Fodrie TY
FAU - Perkins, Sherrie L
AU  - Perkins SL
FAU - Kaiser-Rogers, Kathleen
AU  - Kaiser-Rogers K
LA  - eng
PT  - Journal Article
DEP - 20080618
PL  - Germany
TA  - J Hematop
JT  - Journal of hematopathology
JID - 101491976
PMC - PMC2713480
EDAT- 2009/08/12 09:00
MHDA- 2009/08/12 09:01
PMCR- 2008/06/18
CRDT- 2009/08/12 09:00
PHST- 2008/01/29 00:00 [received]
PHST- 2008/04/24 00:00 [accepted]
PHST- 2009/08/12 09:00 [entrez]
PHST- 2009/08/12 09:00 [pubmed]
PHST- 2009/08/12 09:01 [medline]
PHST- 2008/06/18 00:00 [pmc-release]
AID - 7 [pii]
AID - 10.1007/s12308-008-0007-7 [doi]
PST - ppublish
SO  - J Hematop. 2008 Sep;1(2):77-84. doi: 10.1007/s12308-008-0007-7. Epub 2008 Jun 18.