PMID- 1966987
OWN - NLM
STAT- MEDLINE
DCOM- 19920729
LR  - 20181130
IS  - 0141-2760 (Print)
IS  - 0141-2760 (Linking)
VI  - 31
IP  - 2
DP  - 1990 Feb
TI  - Studies of the acute phase response in experimental serum sickness.
PG  - 69-73
AB  - The acute phase behavior of C-reactive protein (C-RP), the third component of 
      complement (C3) and total haemolytic complement activity was studied during the 
      course of acute serum sickness (ASS) in rabbits. The specific aim was to 
      establish whether the induction protocol caused a significant change in the serum 
      concentration of proteins (such as C3) which could modify the outcome of the 
      disease. ASS was induced by an intravenous (IV) bolus of bovine serum albumin 
      (250 mg/Kg) with or without prior subcutaneous (SC) immunization with 4 mg BSA in 
      complete Freund's adjuvant. Thirty-six animals received a full induction regimen 
      (i.e., both SC and IV BSA). A further six rabbits were given either IV or SC BSA 
      alone, in order to define the basis for acute phase changes observed when both 
      injections were given. Twelve animals received a standard acute phase stimulus 
      with intramuscular (IM) turpentine--2 or 3 ml--as a comparison to the response 
      observed in the experimental animals. Nine, 14 and 24 animals showed a rise in 
      C-RP (i.e., five-fold increase), C3 and haemolytic activity (greater than 25% 
      increase) respectively after a full induction protocol. Eight, nine and five 
      animals showed a comparable rise with IM turpentine. Studies with IV or SC BSA 
      alone showed that the latter was predominantly responsible for the rise in C-RP 
      and haemolytic activity. Specifically, five and four animals respectively showed 
      a significant rise in these two parameters. Three animals showed a rise in C-RP 
      following IV BSA alone.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Martyn, P
AU  - Martyn P
AD  - Department of Medicine, Prince Henry Hospital, Sydney, Australia.
FAU - Charlesworth, J A
AU  - Charlesworth JA
FAU - Peake, P W
AU  - Peake PW
FAU - Pussell, B A
AU  - Pussell BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Scotland
TA  - J Clin Lab Immunol
JT  - Journal of clinical & laboratory immunology
JID - 7808987
RN  - 0 (Complement C3)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - XJ6RUH0O4G (Turpentine)
SB  - IM
MH  - Acute Disease
MH  - *Acute-Phase Reaction
MH  - Animals
MH  - C-Reactive Protein/*analysis
MH  - Complement C3/*analysis
MH  - Glomerulonephritis/etiology
MH  - Hemolysis
MH  - Immune Complex Diseases/*blood/complications
MH  - Immunization
MH  - Rabbits
MH  - Serum Albumin, Bovine
MH  - Serum Sickness/*blood/complications
MH  - Turpentine
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
PST - ppublish
SO  - J Clin Lab Immunol. 1990 Feb;31(2):69-73.