PMID- 1967066
OWN - NLM
STAT- MEDLINE
DCOM- 19921026
LR  - 20141120
IS  - 0141-2760 (Print)
IS  - 0141-2760 (Linking)
VI  - 33
IP  - 2
DP  - 1990 Oct
TI  - Triggering effects of opsonized-IgG antibody on the superoxide release in the 
      phagosome and phagosome-lysosome fusion by pulmonary alveolar macrophages in 
      rabbits.
PG  - 55-9
AB  - We studied the triggering effects of opsonized-IgG antibody on the superoxide 
      (O2-) release and phagosome-lysosome fusion in the phagosome of pulmonary 
      alveolar macrophages (PAM). Both were histochemically assayed in the same cell to 
      enable simultaneous observation, using the qualitative superoxide dismutase 
      (SOD)-inhibitable nitroblue tetrazolium (NBT) reduction test and the acridine 
      orange (AO) method. When PAM from normal rabbit lungs were made to phagocytize 
      the IgG-treated yeasts, the cells showed the enhanced release of O2- which acted 
      directly on the antibody-coated yeasts as an oxidant, and the enhanced 
      phagosome-lysosome fusion that was inhibited by SOD and was closely related to 
      the enhanced release of O2- in the phagosomes. In contrast, neither enhanced O2- 
      release nor phagosome-lysosome fusion was observed in the PAM which phagocytized 
      saline-treated yeasts. These results may suggest that the opsonized-IgG antibody 
      enhances the O2- release in the phagosome, resulting in the enhanced 
      phagosome-lysosome fusion.
FAU - Suga, M
AU  - Suga M
AD  - Kumamoto Rosai Hospital, Japan.
FAU - Ando, M
AU  - Ando M
FAU - Tanaka, F
AU  - Tanaka F
FAU - Araki, S
AU  - Araki S
LA  - eng
PT  - Journal Article
PL  - Scotland
TA  - J Clin Lab Immunol
JT  - Journal of clinical & laboratory immunology
JID - 7808987
RN  - 0 (Immunoglobulin G)
RN  - 0 (Opsonin Proteins)
RN  - 11062-77-4 (Superoxides)
SB  - IM
MH  - Animals
MH  - *Immunoglobulin G
MH  - In Vitro Techniques
MH  - Macrophages, Alveolar/*immunology/*metabolism
MH  - Membrane Fusion/immunology
MH  - Opsonin Proteins
MH  - Phagocytosis
MH  - Phagosomes/immunology/metabolism
MH  - Rabbits
MH  - Superoxides/*metabolism
MH  - Trichosporon/immunology
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
PST - ppublish
SO  - J Clin Lab Immunol. 1990 Oct;33(2):55-9.